Difference between revisions of "UW-Stout/About"

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About lorem ipsum....
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===About the UW-Stout Orphan Gene Project===
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In the spring of 2019, the Intro Cell and Molecular Biology class at the
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[https://uwstout.edu University of Wisconsin -- Stout] began
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[[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a
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[https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]
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background, then screening those knockout strains for phenotypes.  This page organizes the methods and data from this project.
 +
 
 +
For more information, or to request a knock-out strain, please contact Dr. Brian Teague
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at teagueb@uwstout.edu.
 +
 
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===[[UW-Stout/Knockout_Protocol|Knockout protocols]]===
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===Knock-out strains (Spring 2023)===
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[[YMR144W]] [[SSK1]] [[YKL162C]]
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===Knock-out strains (Fall 2022)===
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[[YCR095C]] [[YER076C]] [[YER186C]] [[YHR033W]] [[YJL133C-A]]
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===Knock-out strains (Spring 2022)===
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[[YBL113C]]  [[YCL049C]]  [[YDL109C]]  [[YDR307W]]  [[YGL146C]]
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[[YGR079W]]  [[YHL029C]]  [[YNL058C]]  [[YOR111W]]
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===Knock-out strains (Fall 2021)===
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[[YBR184W]]  [[YBR284W]]  [[YHL012W]]  [[YMR221C]]  [[YPR078C]]
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===Knock-out strains (Spring 2021)===
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[[YBR225W]]  [[YGL138C]]  [[YKL121W]]  [[YLR426W]]  [[YNL018C]]
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[[YOR387C]]
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===Knock-out strains (Spring 2019)===
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[[YCL002C]]  [[YCR100C]]  [[YEL035C]]  [[YFL064C]]  [[YGL235W]] 
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[[YJL043W]]  [[YMR090W]]  [[YPL068C]]
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 +
 
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===Knock-out strains (Fall 2019)===
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[[YAL061W]]  [[YBL086C]]  [[YBL081W]]  [[YBR033W]]  [[YBR138C]]
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[[YBR220C]]  [[YHR048W]]  [[YGL140C]]  [[YJR061W]]  [[YKR005C]]
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===Protocols (Spring 2019)===
 
===Protocols (Spring 2019)===
 
[[UW-Stout/Growth_Curve|Basic growth curve protocol]]
 
[[UW-Stout/Growth_Curve|Basic growth curve protocol]]
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[[UW-Stout/Heat_Shock|Heat Shock]]
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[[UW-Stout/G-418|G-418 Stress]]
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[[UW-Stout/Nitrogen_Starvation|Nitrogen Starvation]]
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[[UW-Stout/Fermentation|Fermentation]]
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[[UW-Stout/Methanol|Methanol]]
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[[UW-Stout/Caffeine |Caffeine]]
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[[UW-Stout/pH|pH Stress]]
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[[UW-Stout/Hydroxyurea|Hydroxyurea]]
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[[UW-Stout/Cold_Shock|Cold Shock]]
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[[UW-Stout/Ultraviolet_Light|Ultraviolet Light]]
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===Protocols (Fall 2020)===
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[[UW-Stout/Growth_Curve|Basic growth curve protocol]]
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[[UW-Stout/Caffeine_FA19|Caffeine]]
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[[UW-Stout/Prednisone_FA19|Prednisone]]
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[[UW-Stout/Hydroxyurea_FA19|Hydroxyurea]]
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[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]
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[[UW-Stout/Hydrogen_peroxide_FA19|Hydrogen peroxide]]
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[[UW_Stout/Cycloheximide_FA19|Cycloheximide]]
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[[UW_Stout/UV_Light_FA19|UV Light]]
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[[UW_Stout/Lithium_chloride_FA19|Lithium Chloride]]
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[[UW_Stout/Formamide_FA19|Formamide]]
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[[UW_Stout/Proline_FA19|Proline utilization]]
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[[UW_Stout/Ethanol_FA19|Ethanol]]
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[[UW_Stout/DMSO_FA19|DMSO]]
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===Protocols (Spring 2021)===
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[[UW-Stout/Growth_Curve_SP21|Basic growth curve protocol]]
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[[UW-Stout/Caffeine_SP21|Caffeine (Group 1, in JHSA 247)]]
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[[UW-Stout/Caffeine__2_SP21|Caffeine (Group 2, in JHSA 267)]]
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[[UW-Stout/Heat_Shock_SP21|Heat shock]]
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[[UW-Stout/Co_Culture_SP21|Competitive co-culture]]
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[[UW-Stout/PMSF_SP21|Phenylmethylsulfonyl fluoride (PMSF)]]
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[[UW-Stout/Ethanol_SP21|Ethanol]]
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[[UW-Stout/UV_Light_SP21|UV Light]]
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[[UW-Stout/Hydroxyurea_SP21|Hydroxyurea]]
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[[UW-Stout/Salt_Concentration_SP21|Salt Concentration (NaCl)]]
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[[UW-Stout/Hydroxychloroquine_SP21|Hydroxychloroquine]]
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[[UW-Stout/Ammonium_Sulfate_SP21|Nitrogen Starvation]]
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[[UW-Stout/Sodium_Hydroxide_SP21|Sodium Hydroxide]]
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[[UW-Stout/Calcofluor_White_SP21|Calcofluor White]]
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===Protocols (Fall 2021)===
 +
 +
[[UW-Stout/Nitrogen_Starvation_FA21|Nitrogen Starvation]]
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[[UW-Stout/Heat_Shock_FA21|Heat shock]]
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[[UW-Stout/Ethanol_FA21|Ethanol]]
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[[UW-Stout/G418_FA21|G-418]]
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[[UW-Stout/Copper_FA21|Copper]]
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[[UW-Stout/Bud_Scars_FA21|Bud scars]]
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[[UW-Stout/pH_FA21|pH]]
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===Protocols (Spring 2022)===
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[[UW-Stout/Growth_Curve_SP22|Basic growth curve protocol]]
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[[UW-Stout/Caffeine_SP22|Caffeine]]
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[[UW-Stout/UV_Light_SP22|UV Light]]
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[[UW-Stout/Formamide_SP22|Formamide]]
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[[UW-Stout/Heat_Shock_SP22|Heat Shock]]
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[[UW-Stout/pH_Acid_SP22|pH (Acid)]]
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[[UW-Stout/Nonionic_Detergent_SP22|Nonionic Detergent]]
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[[UW-Stout/Hydrogen_Peroxide_SP22|Hydrogen Peroxide]]
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[[UW_Stout/Sucrose_Fermentation_SP22|Sucrose Fermentation]]
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[[UW_Stout/D2O_SP22|Duterium Oxide]]
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[[UW_Stout/Nystatin_SP22|Nystatin]]
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===Protocols (Fall 2022)===
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 +
[[UW-Stout/Growth_Curve_FA22|Basic growth curve protocol]]
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 +
[[UW-Stout/Ethanol_FA22|Ethanol]]
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[[UW-Stout/Hydrogen_Peroxide_FA22|Hydrogen Peroxide]]
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[[UW-Stout/Glycerol_FA22|Glycerol media]]
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[[UW-Stout/Ultraviolet_Light_FA22|UV Light]]
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[[UW-Stout/Caffeine_FA22|Caffeine]]
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[[UW-Stout/Bud_Scars_FA22|Bud scars]]
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===Protocols (Spring 2023)===
 +
 +
[[UW-Stout/Growth_Curve_SP23|Basic growth curve protocol]]
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 +
[[UW-Stout/Acids_SP23|pH (Acid)]]
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[[UW-Stout/Bases_SP23|pH (Bases)]]
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[[UW-Stout/Voltage_SP23|Electrical Shock]]
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[[UW-Stout/Heat_shock_SP23|Heat shock]]
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[[UW-Stout/Isopropanol_SP23|Isopropanol]]
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[[UW-Stout/G418_SP23|Genticin]]
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[[UW-Stout/Saline_SP23|Salinity]]
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[[UW-Stout/Glucose_SP23|Glucose toxicity]]
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[[UW-Stout/Fermentation_SP23|Fermentation]]
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[[UW-Stout/Caffeine_SP23|Caffeine]]

Latest revision as of 11:21, 25 April 2023

About the UW-Stout Orphan Gene Project

In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.

For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.

Knockout protocols

Knock-out strains (Spring 2023)

YMR144W SSK1 YKL162C

Knock-out strains (Fall 2022)

YCR095C YER076C YER186C YHR033W YJL133C-A

Knock-out strains (Spring 2022)

YBL113C YCL049C YDL109C YDR307W YGL146C YGR079W YHL029C YNL058C YOR111W

Knock-out strains (Fall 2021)

YBR184W YBR284W YHL012W YMR221C YPR078C

Knock-out strains (Spring 2021)

YBR225W YGL138C YKL121W YLR426W YNL018C YOR387C

Knock-out strains (Spring 2019)

YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C


Knock-out strains (Fall 2019)

YAL061W YBL086C YBL081W YBR033W YBR138C YBR220C YHR048W YGL140C YJR061W YKR005C


Protocols (Spring 2019)

Basic growth curve protocol

Heat Shock

G-418 Stress

Nitrogen Starvation

Fermentation

Methanol

Caffeine

pH Stress

Hydroxyurea

Cold Shock

Ultraviolet Light


Protocols (Fall 2020)

Basic growth curve protocol

Caffeine

Prednisone

Hydroxyurea

Nitrogen starvation

Hydrogen peroxide

Cycloheximide

UV Light

Lithium Chloride

Formamide

Proline utilization

Ethanol

DMSO


Protocols (Spring 2021)

Basic growth curve protocol

Caffeine (Group 1, in JHSA 247)

Caffeine (Group 2, in JHSA 267)

Heat shock

Competitive co-culture

Phenylmethylsulfonyl fluoride (PMSF)

Ethanol

UV Light

Hydroxyurea

Salt Concentration (NaCl)

Hydroxychloroquine

Nitrogen Starvation

Sodium Hydroxide

Calcofluor White

Protocols (Fall 2021)

Nitrogen Starvation

Heat shock

Ethanol

G-418

Copper

Bud scars

pH

Protocols (Spring 2022)

Basic growth curve protocol

Caffeine

UV Light

Formamide

Heat Shock

pH (Acid)

Nonionic Detergent

Hydrogen Peroxide

Sucrose Fermentation

Duterium Oxide

Nystatin

Protocols (Fall 2022)

Basic growth curve protocol

Ethanol

Hydrogen Peroxide

Glycerol media

UV Light

Caffeine

Bud scars

Protocols (Spring 2023)

Basic growth curve protocol

pH (Acid)

pH (Bases)

Electrical Shock

Heat shock

Isopropanol

Genticin

Salinity

Glucose toxicity

Fermentation

Caffeine

Retrieved from "https://wiki.yeastgenome.org/index.php?title=UW-Stout/About&oldid=405786"