UW-Stout/About

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About the UW-Stout Orphan Gene Project

In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.

For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.

Knockout protocols

Knock-out strains (Spring 2019)

YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C


Knock-out strains (Fall 2019)

YAL061W YBL086C YBL081W YBR033W YBR138C YBR220C YHR048W YGL140C YJR061W YKR005C


Protocols (Spring 2019)

Basic growth curve protocol

Heat Shock

G-418 Stress

Nitrogen Starvation

Fermentation

Methanol

Caffeine

pH Stress

Hydroxyurea

Cold Shock

Ultraviolet Light


Protocols (Fall 2020)

Basic growth curve protocol

Caffeine

Prednisone

Hydroxyurea

Nitrogen starvation

Hydrogen peroxide

Cycloheximide

UV Light

Lithium Chloride

Formamide

Proline utilization

Ethanol

DMSO