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|Feature type||ORF, Uncharacterized|
Description of YBR284W: Protein of unknown function; some similarity to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin, and synthetic phenotype with expression of alpha-synuclein; induced in response to hydrostatic pressure; not an essential gene
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
- This yeast strain had strong growth in the control group but was very stressed by the heat shock and showed less clusters/colonies on the heat shocked trial plates. The heat shocked trial plates showed a growth decrease by 4 times.
- For the YBR284W gene, results showed that there was a slight increase in growth on the dish that did not contain NH₄ where there were more colonies of yeast.
- When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain
Cells grown with this gene (YBR284W) knocked out had an average of 1.18 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 114 YBR284W cells counted, 135 bud scars were seen. This indicated that the YBR284W gene slows reproduction rate and thus bud scarring in yeast cells.
The YBR284W yeast strain doubling time without any stress was 174 minutes which is pretty similar to the wild type with 182 minutes. When both strains were stressed by G418 they YBR284W doubling time was 173 minutes and the wild type was 178 minutes. (These times are averaged between three trials) Protocol: 
- The Yeast Strain of YBR284W was not affected in any waty by the 18.5 % Ethanol it still has a steady growth curve
The average doubling time for YBR284W stressed with our CuSO4 solution was 239 minutes, while unstressed was 207. The stressed version grew approximately 14% slower than the unstressed version. The unstressed version appears to have also grown larger overall.
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- Gatbonton T, et al. (2006) Telomere length as a quantitative trait: genome-wide survey and genetic mapping of telomere length-control genes in yeast. PLoS Genet 2(3):e35 SGD PMID 16552446
- Holmstrom K, et al. (1994) The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae. Yeast 10 Suppl A:S47-62 SGD PMID 8091861
- Iwahashi H, et al. (2003) Piezophysiology of genome wide gene expression levels in the yeast Saccharomyces cerevisiae. Extremophiles 7(4):291-8 SGD PMID 12910389
- Maxwell PH and Curcio MJ (2007) Host factors that control long terminal repeat retrotransposons in Saccharomyces cerevisiae: implications for regulation of mammalian retroviruses. Eukaryot Cell 6(7):1069-80 SGD PMID 17496126
- Saint-Marc C, et al. (2009) Phenotypic consequences of purine nucleotide imbalance in Saccharomyces cerevisiae. Genetics 183(2):529-38, 1SI-7SI SGD PMID 19635936
- Willingham S, et al. (2003) Yeast genes that enhance the toxicity of a mutant huntingtin fragment or alpha-synuclein. Science 302(5651):1769-72 SGD PMID 14657499
- Xie MW, et al. (2005) Insights into TOR function and rapamycin response: chemical genomic profiling by using a high-density cell array method. Proc Natl Acad Sci U S A 102(20):7215-20 SGD PMID 15883373
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