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|Feature type||ORF, Uncharacterized|
Description of YOR111W: Putative protein of unknown function
- 1 Community Commentary
- 1.1 UW Stout/D2O SP22
- 1.2 UW Stout/Sucrose Fermentation SP22
- 1.3 UW-Stout/UV Light SP22
- 1.4 UW-Stout/Heat Shock SP22
- 1.5 UW-Stout/Caffeine SP22
- 1.6 UW-Stout/Hydrogen Peroxide SP22
- 1.7 UW Stout/Nystatin SP22
- 1.8 UW-Stout/Formamide SP22
- 1.9 Results
- 1.10 Interpretation
- 2 References
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The Knock Out Yeast Strain YOR111W (A3) was unaffected by the 35% dilution of the D2O. Due to the fact that is stayed in the Optical density of about 0.4-0.5 OD 600.
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 80% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.
From the data gathered, there was a medium positive effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were 275% more resistant to heat shock than the control.
- BY4735(wild type yeast) and YOR111W(knockout yeast gene) growth after being subjected to 4mM caffeine solution.
As can be seen in the growth curve, YOR111W is more growth resistant to caffeine than the Wild-Type Yeast, boasting a Optical Density 600 measurement almost 30% higher.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to hydrogen peroxide.
BY4735 and YOR111W after being exposed to 0.065% dilution of hydrogen peroxide solution.
The growth curve suggests that YOR111W may have some level of decreased sensitivity to oxidative stress. Both the knockout and control exhibited more growth than wild type cells in the same scenarios. While the difference in range for the cells treated with hydrogen peroxide is not large, it is at least notable as the knockout stayed above wild type consistently.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
Based on the growth curves, we can see that the YOR111W control is growing at a faster rate than both the wild type control and the stressed wild type. However, the YOR111W being treated with Nystatin is growing at a slower rate than both wild type control and stressed wild type. This indicates that the removal of the YOR111W gene lessens the cells resistance to Nystatin.
- 3% formamide line graph: x-axis= growing time (min); y-axis= optical density 600
- Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
- Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. In the experiment, some of the transformed yeast cells were heavily effected by the formamide, including the YOR111W strain. The YOR111W strain had doubling times significantly faster than the wild yeast cell, with a doubling time of 637.92 min. It can be concluded that the transformed yeast cell, YOR111W, had a unique response to the formamide stress test used in the experiment.
See Help:References on how to add references
- SGD (2006) No information available SGD PMID
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 0.001% concentrated NLS. (Nonionic detergent)
-According to the graph above, we can see that the KO yeast cell YOR111W follow a smaller sensitivity to the stress than the Wild type strain.
-The KO with NLS peaks a lot later than the KO w/o NLS
-We can then conclude that the knocked-out gene in YOR111W doesn't have a significant impact on the growth of the yeast cell.
Knocked out gene seems to have some effect on cell growth in pH 7 environment based on the flattening of the growth curve. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.
The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.