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|Feature type||ORF, Uncharacterized|
Description of YCL049C: Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene
- 1 Community Commentary
- 1.1 UW Stout/D2O SP22
- 1.2 UW Stout/Sucrose Fermentation SP22
- 1.3 UW-Stout/UV Light SP22
- 1.4 UW-Stout/Heat Shock SP22
- 1.5 UW-Stout/Caffeine SP22
- 1.6 UW-Stout/Hydrogen Peroxide SP22
- 1.7 UW Stout/Nystatin SP22
- 1.8 UW-Stout/Formamide SP22
- 1.9 Results
- 1.10 Interpretation
- 2 References
- 3 UW-Stout/Nonionic Detergent SP22
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
With YCL049C (A7) we see that this Knock-Out Yeast Strain is unaffected by the 35% dilution of D2O. You can see that the growth curve hits it limit at about 0.5-0.6 approximately.
This gene produced 55.61% of the amount of ethanol that the wild type produced.
INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.
From the data gathered, there was no effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were within the margin of error to be the same as the control cells.
- BY4735(wild type yeast) and YCL049C(knockout yeast gene) growth after being subjected to 4mM caffeine solution.
As can be seen from the growth curve, YCL049C showed a smaller response to caffeine than the Wild-Type yeast. The difference in response is measured at a 33% difference in Optical Density 600.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to hydrogen peroxide.
BY4735 and YCL049C after being exposed to 0.065% dilution of hydrogen peroxide solution.
From the growth curve, it can be observed that while the sample of the knockout strain was inhibited when treated with hydrogen peroxide, data range was very close to the control. This suggests that YCL049C may simply grow slower than wild type cells.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 1µg/ml Nystatin solution.
Based on the growth curves, we can see that the YCLO49C control is growing at a faster rate than both the wild type control and the stressed wild type. However, the YCLO49C being treated with Nystatin is growing at a slower rate than both wild type control and stressed wild type. This indicates that the removal of the YCLO49C gene lessens the cells resistance to Nystatin.
- 3% formamide line graph: x-axis= growing time (min); y-axis= optical density 600
- Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
- Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)
The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. Where some of the transformed yeast cells were heavily effected by the formamide, the YCL049C strain had doubling times just slightly slower than the wild yeast cell, with a doubling time of 1507.16 min. It can be concluded that the transformed yeast cell, YCL049C, had a slight response to the formamide stress test used in the experiment in comparison to the wild yeast cell.
See Help:References on how to add references
- Terashima H, et al. (2002) Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae. Curr Genet 40(5):311-6 SGD PMID 11935221
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As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 0.001% concentrated NLS. (Nonionic detergent)
-According to the graph above, we can see that the KO yeast cell YCL049C follow a smaller sensitivity to the stress than the Wild type strain.
-We can then conclude that the knocked-out gene in YCL049C doesn't have a significant impact on the growth of the yeast cell.
Knocked out gene seems to have some effect on cell growth in pH 7 environment based on the flattening of the growth curve. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.
The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.