YDL109C

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Systematic name YDL109C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr IV:267201..265258
Primary SGDID S000002267


Description of YDL109C: Putative lipase; involved in lipid metabolism; YDL109C is not an essential gene[1]




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Community Commentary

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UW-Stout/Heat Shock SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.

Results

BY4735 Control YDL109C BY4735 Control Graph YDL109C Graph

Interpretation

From the data gathered, there was a large positive effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were 347% more resistant to heat shock than the control.

UW Stout/Sucrose Fermentation SP22

Gene Glucose Fructose Ethanol
Standard solution 2.0000 0.2000 2.0000
YDl109C 0.3800 0.3933 0.3430
YGL140C 0.2212 0.2685 0.1867
YOR111W 0.3332 0.3598 0.1343
YHL029C 0.3870 0.2368 0.1151
YDR307W 0.4366 0.2487 0.0606
YNL058C 0.2710 0.3056 0.1577
YCL049C 0.4078 0.3052 0.1969
YGR079W 0.4042 0.1589 0.0080
YBL113C 0.3498 0.2012 0.1434
BY4735 0.3171 0.3084 0.3541

Interpretation

This gene produced 96.87% of the amount of ethanol that the wild type produced.

UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene1.jpg


INTERPERTATION In the graph and photos above you can see that exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 90% of yeast cell cultures, in comparison to its control counterpart which was the the same gene and amount of cells, just was not exposed to UV Light.

UW-Stout/D2O SP22

4.28.2022 1.png

The YDL109C (A1) Knock Out Yeast Strain was a little more in the middle spectrum of results with being a little more resistant to the 35% dilution of D2O. The range it stays in as a maximum OD 600 is 0.3-0.4. With the growth curve decaying at the end at about 16 hours.

UW-Stout/Caffeine SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 4mM of caffeine following this protocol.

Results

BY4735(wild type yeast) and YDL109C(knockout yeast gene) growth after being subjected to 4mM caffeine solution.

YDL109C.jpeg

Interpretation

As can be seen in the growth curve, YDL109C shows a similar response to caffeine as the Wild-Type yeast, however, YDL109C may be slightly more resistant to caffeine.

DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically higher expression in phosphorus limited chemostat cultures versus phosphorus excess. [2] [3]

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.





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UW-Stout/Hydrogen Peroxide SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to hydrogen peroxide.

Results

BY4735 and YDL109C after being exposed to 0.065% dilution of hydrogen peroxide solution.

YDL109Cperosm.png

Interpretation

From the growth curve, we can see that YDL109C was inhibited when compared to it's own control, but was still consistent with the rate of growth for unstressed wild type cells. YDL109C may have a higher growth celling the wild strain overall.


UW-Stout/pH(acid) SP22

Strain1.jpeg 1 Knocked out gene seems to have some effect on cell growth in pH 7 environment based on the flattening of the growth curve. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve. Please see protocol for specific quantitative doubling time results.

wild.jpeg The wild-type strain was used as a control in this experiment; no genes were knocked out, it just represents how the experiment ran on the knocked-out gene strains affects a "typical" cell growth curve. Inclusion of this data on each knock-out strain growth curve graph made for difficult interpretation, so it has been separated. Please refer to this graph as a control when viewing the knock-out strain growth curve graph. Spikes in stressed line are to be ignored; they occurred due to clumping of cells during analysis. We are focusing on the general linear trend of the growth curve.

UW Stout/Nystatin SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 1µg/ml Nystatin solution.

Results

YDL-T1.jpg

YDL-T2.jpg

The growth rates of the experimental and control YDL146C strain, when compared to that of the wild type yeast, were similar enough not to indicate a difference in Nystatin tolerance when the YDL146C gene was knocked out.


UW-Stout/Formamide SP22

Results

wildyeast.jpg yeast.jpg doublingtimestable.jpg


  • 3% formamide line graph: x-axis= growing time (min); y-axis= optical density 600
  • Trial 2 line graph: x-axis= growing time (min); y-axis= optical density 600; key= yeast strains and corresponding color to the individual lines in the line graph
  • Average doubling time bar graph: x-axis= yeast strains and corresponding colors to the trial 2 line graph; y-axis= average doubling time (min)

Interpretation

The wild yeast cell treated with 3% formamide had a doubling time of 876.69 min. The 3% formamide solution added to nine transformed yeast cells was then compared to the wild yeast cell using the computed doubling times to see the effects of the formamide. Where some of the transformed yeast cells were heavily effected by the formamide, the YDL109C strain had a doubling time that was close to the doubling time of the wild yeast cell, with a doubling time of 923.43 min. It can be concluded that the transformed yeast cell, YDL109C, had little to no response to the formamide stress test used in the experiment.

References

See Help:References on how to add references

  1. Daum G, et al. (1999) Systematic analysis of yeast strains with possible defects in lipid metabolism. Yeast 15(7):601-14 SGD PMID 10341423
  2. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  3. submitted by Viktor Boer on 2003-07-25

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UW-Stout/Nonionic Detergent SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 0.001% concentrated NLS. (Nonionic detergent)

YDL109C.png

Interpretations:

-According to the graph above, we can see that the KO yeast cell YDL109C follow a smaller sensitivity to the stress than the Wild type strain.

-The KO with NLS peaks a lot later than the KO w/o NLS

-We can then conclude that the knocked-out gene in YDL109C doesn't have a significant impact on the growth of the yeast cell.