Difference between revisions of "UW-Stout/About"
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− | About | + | ===About the UW-Stout Orphan Gene Project=== |
+ | In the spring of 2019, the Intro Cell and Molecular Biology class at the | ||
+ | [https://uwstout.edu University of Wisconsin -- Stout] began | ||
+ | [[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a | ||
+ | [https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain] | ||
+ | background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project. | ||
+ | |||
+ | For more information, or to request a knock-out strain, please contact Dr. Brian Teague | ||
+ | at teagueb@uwstout.edu. | ||
+ | |||
+ | ===[[UW-Stout/Knockout_Protocol|Knockout protocols]]=== | ||
+ | |||
+ | ===Knock-out strains (Spring 2021)=== | ||
+ | [[YBR225W]] [[YGL138C]] [[YKL121W]] [[YLR426W]] [[YNL018C]] | ||
+ | [[YOR387C]] | ||
+ | |||
+ | ===Knock-out strains (Spring 2019)=== | ||
+ | [[YCL002C]] [[YCR100C]] [[YEL035C]] [[YFL064C]] [[YGL235W]] | ||
+ | [[YJL043W]] [[YMR090W]] [[YPL068C]] | ||
+ | |||
+ | |||
+ | ===Knock-out strains (Fall 2019)=== | ||
+ | [[YAL061W]] [[YBL086C]] [[YBL081W]] [[YBR033W]] [[YBR138C]] | ||
+ | [[YBR220C]] [[YHR048W]] [[YGL140C]] [[YJR061W]] [[YKR005C]] | ||
+ | |||
===Protocols (Spring 2019)=== | ===Protocols (Spring 2019)=== | ||
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[[UW-Stout/Caffeine |Caffeine]] | [[UW-Stout/Caffeine |Caffeine]] | ||
+ | |||
+ | [[UW-Stout/pH|pH Stress]] | ||
+ | |||
+ | [[UW-Stout/Hydroxyurea|Hydroxyurea]] | ||
+ | |||
+ | [[UW-Stout/Cold_Shock|Cold Shock]] | ||
+ | |||
+ | [[UW-Stout/Ultraviolet_Light|Ultraviolet Light]] | ||
+ | |||
+ | |||
+ | ===Protocols (Fall 2020)=== | ||
+ | [[UW-Stout/Growth_Curve|Basic growth curve protocol]] | ||
+ | |||
+ | [[UW-Stout/Caffeine_FA19|Caffeine]] | ||
+ | |||
+ | [[UW-Stout/Prednisone_FA19|Prednisone]] | ||
+ | |||
+ | [[UW-Stout/Hydroxyurea_FA19|Hydroxyurea]] | ||
+ | |||
+ | [[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]] | ||
+ | |||
+ | [[UW-Stout/Hydrogen_peroxide_FA19|Hydrogen peroxide]] | ||
+ | |||
+ | [[UW_Stout/Cycloheximide_FA19|Cycloheximide]] | ||
+ | |||
+ | [[UW_Stout/UV_Light_FA19|UV Light]] | ||
+ | |||
+ | [[UW_Stout/Lithium_chloride_FA19|Lithium Chloride]] | ||
+ | |||
+ | [[UW_Stout/Formamide_FA19|Formamide]] | ||
+ | |||
+ | [[UW_Stout/Proline_FA19|Proline utilization]] | ||
+ | |||
+ | [[UW_Stout/Ethanol_FA19|Ethanol]] | ||
+ | |||
+ | [[UW_Stout/DMSO_FA19|DMSO]] | ||
+ | |||
+ | |||
+ | ===Protocols (Spring 2021)=== | ||
+ | [[UW-Stout/Growth_Curve_SP21|Basic growth curve protocol]] | ||
+ | |||
+ | [[UW-Stout/Caffeine_SP21|Caffeine (Group 1, in JHSA 247)]] | ||
+ | |||
+ | [[UW-Stout/Caffeine__2_SP21|Caffeine (Group 2, in JHSA 267)]] | ||
+ | |||
+ | [[UW-Stout/Heat_Shock_SP21|Heat shock]] | ||
+ | |||
+ | [[UW-Stout/Co_Culture_SP21|Competitive co-culture]] | ||
+ | |||
+ | [[UW-Stout/PMSF_SP21|Phenylmethylsulfonyl fluoride (PMSF)]] | ||
+ | |||
+ | [[UW-Stout/Ethanol_SP21|Ethanol]] | ||
+ | |||
+ | [[UW-Stout/UV_Light_SP21|UV Light]] | ||
+ | |||
+ | [[UW-Stout/Hydroxyurea_SP21|Hydroxyurea]] | ||
+ | |||
+ | [[UW-Stout/Salt_Concentration_SP21|Salt Concentration (NaCl)]] | ||
+ | |||
+ | [[UW-Stout/Hydroxychloroquine_SP21|Hydroxychloroquine]] | ||
+ | |||
+ | [[UW-Stout/Ammonium_Sulfate_SP21|Nitrogen Starvation]] | ||
+ | |||
+ | [[UW-Stout/Sodium_Hydroxide_SP21|Sodium Hydroxide]] | ||
+ | |||
+ | [[UW-Stout/Calcofluor_White_SP21|Calcofluor White]] |
Revision as of 20:39, 30 April 2021
Contents
About the UW-Stout Orphan Gene Project
In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.
For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.
Knockout protocols
Knock-out strains (Spring 2021)
YBR225W YGL138C YKL121W YLR426W YNL018C YOR387C
Knock-out strains (Spring 2019)
YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C
Knock-out strains (Fall 2019)
YAL061W YBL086C YBL081W YBR033W YBR138C YBR220C YHR048W YGL140C YJR061W YKR005C
Protocols (Spring 2019)
Protocols (Fall 2020)
Protocols (Spring 2021)
Caffeine (Group 1, in JHSA 247)
Caffeine (Group 2, in JHSA 267)