UW-Stout/Ultraviolet Light

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  1. Three days before the experiment, streak knockout strains onto YPD plates.
  2. One day before the experiment, pick a colony from each strain into 5 ml YPD broth in a disposable test-tube. Incubate on the roller drum at 30°C overnight.
  3. The morning of the experiment, dilute each culture to an OD600 of 0.2 in YPD broth. Return to the drum roller for between 30 and 90 minutes.
  4. Transfer 33 µl to a well in the Culture Dish.
  5. Set up the UV light Exposure System as follows:
    • 400 watts
    • time increments
    • place in the incubator in dark for 48 hours


UV Control.jpeg UV 5 seconds.jpeg UV 10 and 15 Seconds.jpeg UV 20 and 50 Seconds.jpeg UV 60 and 100 Seconds.jpeg UV 500 and 900 Seconds.jpeg UV 700 seconds and 900 seconds.jpeg

We chose to use the control of 700 seconds given the fact that it had cell death but a small amount of colonies present.