Difference between revisions of "UW-Stout/About"
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===About the UW-Stout Orphan Gene Project=== | ===About the UW-Stout Orphan Gene Project=== | ||
− | + | In the spring of 2019, the Intro Cell and Molecular Biology class at the | |
− | [https://uwstout.edu University of Wisconsin -- Stout] | + | [https://uwstout.edu University of Wisconsin -- Stout] began |
[[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a | [[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a | ||
[https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain] | [https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain] | ||
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For more information, or to request a knock-out strain, please contact Dr. Brian Teague | For more information, or to request a knock-out strain, please contact Dr. Brian Teague | ||
at teagueb@uwstout.edu. | at teagueb@uwstout.edu. | ||
+ | |||
+ | ===[[UW-Stout/Knockout_Protocol|Knockout protocols]]=== | ||
===Knock-out strains (Spring 2019)=== | ===Knock-out strains (Spring 2019)=== | ||
[[YCL002C]] [[YCR100C]] [[YEL035C]] [[YFL064C]] [[YGL235W]] | [[YCL002C]] [[YCR100C]] [[YEL035C]] [[YFL064C]] [[YGL235W]] | ||
[[YJL043W]] [[YMR090W]] [[YPL068C]] | [[YJL043W]] [[YMR090W]] [[YPL068C]] | ||
+ | |||
+ | |||
+ | ===Knock-out strains (Fall 2019)=== | ||
+ | [[YAL061W]] [[YBL086C]] [[YBL081W]] [[YBR033W]] [[YBR138C]] | ||
+ | [[YBR220C]] [[YHR048W]] [[YGL140C]] [[YJR061W]] [[YKR005C]] | ||
+ | |||
===Protocols (Spring 2019)=== | ===Protocols (Spring 2019)=== | ||
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[[UW-Stout/Hydroxyurea|Hydroxyurea]] | [[UW-Stout/Hydroxyurea|Hydroxyurea]] | ||
+ | |||
+ | [[UW-Stout/Cold_Shock|Cold Shock]] | ||
+ | |||
+ | [[UW-Stout/Ultraviolet_Light|Ultraviolet Light]] | ||
+ | |||
+ | |||
+ | ===Protocols (Fall 2020)=== | ||
+ | [[UW-Stout/Growth_Curve|Basic growth curve protocol]] | ||
+ | |||
+ | [[UW-Stout/Caffeine_FA19|Caffeine]] | ||
+ | |||
+ | [[UW-Stout/Prednisone_FA19|Prednisone]] | ||
+ | |||
+ | [[UW-Stout/Hydroxyurea_FA19|Hydroxyurea]] | ||
+ | |||
+ | [[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]] | ||
+ | |||
+ | [[UW-Stout/Hydrogen_peroxide_FA19|Hydrogen peroxide]] | ||
+ | |||
+ | [[UW_Stout/Cycloheximide_FA19|Cycloheximide]] | ||
+ | |||
+ | [[UW_Stout/UV_Light_FA19|UV Light]] | ||
+ | |||
+ | [[UW_Stout/Lithium_chloride_FA19|Lithium Chloride]] | ||
+ | |||
+ | [[UW_Stout/Formamide_FA19|Formamide]] | ||
+ | |||
+ | [[UW_Stout/Proline_FA19|Proline utilization]] | ||
+ | |||
+ | [[UW_Stout/Ethanol_FA19|Ethanol]] | ||
+ | |||
+ | [[UW_Stout/DMSO_FA19|DMSO]] |
Revision as of 13:52, 18 December 2019
Contents
About the UW-Stout Orphan Gene Project
In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.
For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.
Knockout protocols
Knock-out strains (Spring 2019)
YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C
Knock-out strains (Fall 2019)
YAL061W YBL086C YBL081W YBR033W YBR138C YBR220C YHR048W YGL140C YJR061W YKR005C
Protocols (Spring 2019)