Difference between revisions of "UW-Stout/About"
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===[[UW-Stout/Knockout_Protocol|Knockout protocols]]=== | ===[[UW-Stout/Knockout_Protocol|Knockout protocols]]=== | ||
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+ | ===Knock-out strains (Fall 2021)=== | ||
+ | [[YBR184W]] [[YBR284W]] [[YHL012W]] [[YMR221C]] [[YPR078C]] | ||
===Knock-out strains (Spring 2021)=== | ===Knock-out strains (Spring 2021)=== | ||
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[[UW-Stout/Calcofluor_White_SP21|Calcofluor White]] | [[UW-Stout/Calcofluor_White_SP21|Calcofluor White]] | ||
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+ | ===Protocols (Fall 2021)=== | ||
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+ | [[UW-Stout/Nitrogen_Starvation_FA21|Nitrogen Starvation]] | ||
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+ | [[UW-Stout/Heat_Shock_FA21|Heat shock]] | ||
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+ | [[UW-Stout/Ethanol_FA21|Ethanol]] | ||
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+ | [[UW-Stout/G418_FA21|G-418]] | ||
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+ | [[UW-Stout/Copper_FA21|Copper]] | ||
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+ | [[UW-Stout/Bud_Scars_FA21|Bud scars]] | ||
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+ | [[UW-Stout/pH_FA21|pH]] |
Revision as of 12:25, 2 December 2021
Contents
[hide]About the UW-Stout Orphan Gene Project
In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.
For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.
Knockout protocols
Knock-out strains (Fall 2021)
YBR184W YBR284W YHL012W YMR221C YPR078C
Knock-out strains (Spring 2021)
YBR225W YGL138C YKL121W YLR426W YNL018C YOR387C
Knock-out strains (Spring 2019)
YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C
Knock-out strains (Fall 2019)
YAL061W YBL086C YBL081W YBR033W YBR138C YBR220C YHR048W YGL140C YJR061W YKR005C
Protocols (Spring 2019)
Protocols (Fall 2020)
Protocols (Spring 2021)
Caffeine (Group 1, in JHSA 247)
Caffeine (Group 2, in JHSA 267)
Phenylmethylsulfonyl fluoride (PMSF)