Difference between revisions of "UW-Stout/About"
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===[[UW-Stout/Knockout_Protocol|Knockout protocols]]=== | ===[[UW-Stout/Knockout_Protocol|Knockout protocols]]=== | ||
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+ | ===Knock-out strains (Spring 2021)=== | ||
+ | [[YBR225W]] [[YGL138C]] [[YKL121W]] [[YLR426W]] [[YNL018C]] | ||
+ | [[YOR387C]] | ||
===Knock-out strains (Spring 2019)=== | ===Knock-out strains (Spring 2019)=== | ||
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[[UW_Stout/DMSO_FA19|DMSO]] | [[UW_Stout/DMSO_FA19|DMSO]] | ||
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+ | ===Protocols (Spring 2021)=== | ||
+ | [[UW-Stout/Growth_Curve_SP21|Basic growth curve protocol]] | ||
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+ | [[UW-Stout/Caffeine_SP21|Caffeine]] | ||
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+ | [[UW-Stout/Heat_Shock_SP21|Heat shock]] | ||
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+ | [[UW-Stout/Co_Culture_SP21|Competitive co-culture]] | ||
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+ | [[UW-Stout/PMSF_SP21|Phenylmethylsulfonyl fluoride (PMSF)]] | ||
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+ | [[UW-Stout/Ethanol_SP21|Ethanol]] | ||
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+ | [[UW-Stout/UV_Light_SP21|UV Light]] | ||
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+ | [[UW-Stout/Hydroxyurea_SP21|Hydroxyurea]] |
Revision as of 08:51, 6 April 2021
Contents
About the UW-Stout Orphan Gene Project
In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.
For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.
Knockout protocols
Knock-out strains (Spring 2021)
YBR225W YGL138C YKL121W YLR426W YNL018C YOR387C
Knock-out strains (Spring 2019)
YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C
Knock-out strains (Fall 2019)
YAL061W YBL086C YBL081W YBR033W YBR138C YBR220C YHR048W YGL140C YJR061W YKR005C
Protocols (Spring 2019)
Protocols (Fall 2020)