Difference between revisions of "UW-Stout/About"

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===About the UW-Stout Orphan Gene Project===
 
===About the UW-Stout Orphan Gene Project===
Starting in the spring of 2019, the Intro Cell and Molecular Biology class at the
+
In the spring of 2019, the Intro Cell and Molecular Biology class at the
[https://uwstout.edu University of Wisconsin -- Stout] has been
+
[https://uwstout.edu University of Wisconsin -- Stout] began
 
[[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a  
 
[[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a  
 
[https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]
 
[https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]

Revision as of 07:16, 10 May 2019

About the UW-Stout Orphan Gene Project

In the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.

For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.

Knock-out strains (Spring 2019)

YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C

Protocols (Spring 2019)

Basic growth curve protocol

Heat Shock

G-418 Stress

Nitrogen Starvation

Fermentation

Methanol

Caffeine

pH Stress

Hydroxyurea

Cold Shock

Ultraviolet Light