Difference between revisions of "YPR078C"

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The YPR078C yeast strain doubling time without any stress was 275 minutes which is much slower than the wild type with 182 minutes. When both strains were stressed by G418 they YPR078C doubling time was 257 minutes and the wild type was 178 minutes. (These times are averaged between three trials)
 
The YPR078C yeast strain doubling time without any stress was 275 minutes which is much slower than the wild type with 182 minutes. When both strains were stressed by G418 they YPR078C doubling time was 257 minutes and the wild type was 178 minutes. (These times are averaged between three trials)
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Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]]
 
Protocol [[https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]]
  

Revision as of 15:37, 20 December 2021

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Systematic name YPR078C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XVI:698265..697147
Primary SGDID S000006282


Description of YPR078C: Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible[1][2]




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Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Heat Shock FA21

Heat Shock.jpeg

YPRO78C (D)

  • The trials showed very consistent results when heat shocked. The growth rate was consistent but just less clusters than the control plate.

Nitrogen Starvation

YPR078C Nitrogen21.jpg

  • In the YPR078C gene, there is a slight increase in growth on the plate that does not contain NH₄. Overall, there is no sign that this particular strain did anything significant to the genes; therefore, makes it resistant to the stress it's being put into.

UW-Stout/Bud Scars FA21

Cells grown with this gene (YPR078C) knocked out had an average of 1.30 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 109 YPR078C cells counted, 142 bud scars were seen. This indicated that the YPR078C gene slows reproduction rate and thus bud scarring in yeast cells.

pH

pH knockout.jpg

YPR078C

  • When this strain was exposed to a citric acid buffer with a pH of 2.6. At first, the yeast growth appeared stunted and then began to slowly trend upward

Protocol [[1]]


UW-Stout/G418 FA21

YPR078C Vs BY4735 G418.png

The YPR078C yeast strain doubling time without any stress was 275 minutes which is much slower than the wild type with 182 minutes. When both strains were stressed by G418 they YPR078C doubling time was 257 minutes and the wild type was 178 minutes. (These times are averaged between three trials) Protocol [[2]]






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References

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  1. ↑ Perkins EL, et al. (1999) Yeast and human genes that affect the Escherichia coli SOS response. Proc Natl Acad Sci U S A 96(5):2204-9 SGD PMID 10051619
  2. ↑ Sakaki K, et al. (2003) Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae. J Biochem 134(3):373-84 SGD PMID 14561723

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