Difference between revisions of "YMR221C"
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Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YMR221C cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells. | Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YMR221C cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells. | ||
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| + | ===[[UW-Stout/pH_FA21|pH]]=== | ||
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| + | [[File:pH_knockout.jpg]] | ||
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| + | XXX123X | ||
| + | *When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain | ||
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Revision as of 14:42, 14 December 2021
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| Systematic name | YMR221C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XIII:715445..713931 |
| Primary SGDID | S000004834 |
Description of YMR221C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy[1][2]
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Contents
Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Heat Shock FA21
YMR221C (C)
- When stressing this strain of yeast, there was not much of a growth difference. The growth rate slowed down once heat shocked.
Nitrogen Starvation
- In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.
UW-Stout/Bud Scars FA21
Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YMR221C cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells.
pH
XXX123X
- When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain
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References
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- ↑ Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
- ↑ Tarassov K, et al. (2008) An in vivo map of the yeast protein interactome. Science 320(5882):1465-70 SGD PMID 18467557
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