YFL064C

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Systematic name	YFL064C
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr VI:3846..3322
Primary SGDID	S000001830

Description of YFL064C: Putative protein of unknown function

Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YFL064C on synthetic defined media (SDC).

Growth of YFL064C on omission media that lacks nitrogen.

Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

Growth Curve

In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.

G-418 Stress

In the BY4735 background, knocking out the YFL064C strain seem to have a different kind of effect, where the YFL064C growth was slowed down but grew faster than the BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YFL064C knock-out strain's doubling time was 142 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YFL064C knock-out strain's doubling time was 100 minutes.

Methanol Sensitivity

The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.

[1]

Heat Shock

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication.

Fermentation

Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Caffeine Sensitivity

Protocol above, results show very different initial reactions, pace of growth very different, but ended at about the same pace.

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Stressing with pH

pH stress protocol link [3]

The wild type strain had a significant difference compared to the yeast strain YFL064C and when we stressed it out at the pH of 4. It displayed that it was more resilient to this particular pH and grew happily than the YFL064C yeast strain did.

Cold Shock Sensitivity

The Results from this graph have proven that compared to the wild type there was no significant change in the doubling time. This has proven that its neither more or less sensitive in comparison to the wild type.

References

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