Difference between revisions of "YFL064C"

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{{UW-Stout}}
 
{{UW-Stout}}
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===[[UW-Stout/Sensitivity To Nitrogen Starvation]]===
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[[File: YFL064C_growth plate.JPG|thumb|Growth of YFL064C on synthetic defined media (SDC).]]
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[[File: YFL064C_starvation plate.JPG|thumb|Growth of YFL064C on omission media that lacks nitrogen.]]
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[[File: YFL064C_revival plate.JPG|thumb|Growth of transferred BY4735 on Synthetic defined media.]]
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Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
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===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===
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[[File:YFL064C uv light.jpg|300 px]]
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[[File:BY4735(wild) uv light.jpg|300 px]]
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YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
  
 
===[[UW-Stout/Growth_Curve|Growth Curve]]===
 
===[[UW-Stout/Growth_Curve|Growth Curve]]===
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In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes.  However, the knock-out strain did reach a substantially higher OD600 before saturating.
 
In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes.  However, the knock-out strain did reach a substantially higher OD600 before saturating.
  
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===[[UW-Stout/G-418 | G-418 Stress]]===
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[[File:image007.png]]
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In the BY4735 background, knocking out the YFL064C strain seem to have a different kind of effect, where the YFL064C growth was slowed down but grew faster than the BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YFL064C knock-out strain's doubling time was 142 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YFL064C knock-out strain's doubling time was 100 minutes.
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===Methanol Sensitivity===
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The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.
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[https://wiki.yeastgenome.org/index.php/UW-Stout/Methanol]
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[[File:YFL064C_sensitivity_Methanol.png]]
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===Heat Shock===
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[[File:YFL064C Heat Shock.PNG]]
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Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.
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===Stressing with Hydroxyurea===
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[[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]]
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-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication.
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[[file:HU_YFL064C.png|left]]
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===Fermentation===
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Fermentation protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]
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- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
  
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[[File:Fer YFL064C.PNG]]
  
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 
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J Biol Chem 278(5):3265-74</ref>
 
J Biol Chem 278(5):3265-74</ref>
 
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===Caffeine Sensitivity===
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[[File:YFL064C.jpg|YFL064C VS BY4735]]
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Protocol above, results show very different initial reactions, pace of growth very different, but ended at about the same pace.
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----
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===Stressing with pH===
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pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
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The wild type strain had a significant difference compared to the yeast strain YFL064C and when we stressed it out at the pH of 4. It displayed that it was more resilient to this particular pH and grew happily than the YFL064C yeast strain did.
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[[File:pH-YFL064C.PNG]]
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===Cold Shock Sensitivity===
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[[File:YFL064CBB.png]]
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Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
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YFL064C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YFL064C.
  
 
==References==
 
==References==
 
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Latest revision as of 18:50, 9 May 2019

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Systematic name YFL064C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VI:3846..3322
Primary SGDID S000001830


Description of YFL064C: Putative protein of unknown function[1]




Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YFL064C on synthetic defined media (SDC).
Growth of YFL064C on omission media that lacks nitrogen.
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.


Ultraviolet Sensitivity

YFL064C uv light.jpg BY4735(wild) uv light.jpg

YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

Growth Curve

yfl064c growth.png

In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.

G-418 Stress

image007.png

In the BY4735 background, knocking out the YFL064C strain seem to have a different kind of effect, where the YFL064C growth was slowed down but grew faster than the BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YFL064C knock-out strain's doubling time was 142 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YFL064C knock-out strain's doubling time was 100 minutes.

Methanol Sensitivity

The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.

[1]

YFL064C sensitivity Methanol.png


Heat Shock

YFL064C Heat Shock.PNG

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication.

HU YFL064C.png

Fermentation

Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Fer YFL064C.PNG


Caffeine Sensitivity

YFL064C VS BY4735 

Protocol above, results show very different initial reactions, pace of growth very different, but ended at about the same pace.

Stressing with pH

pH stress protocol link [3]


The wild type strain had a significant difference compared to the yeast strain YFL064C and when we stressed it out at the pH of 4. It displayed that it was more resilient to this particular pH and grew happily than the YFL064C yeast strain did.

pH-YFL064C.PNG

Cold Shock Sensitivity

YFL064CBB.png


Protocol: Cold Shock


YFL064C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YFL064C.

References

See Help:References on how to add references

  1. ↑ SGD (2006) No information available SGD PMID

See Help:Categories on how to add the wiki page for this gene to a Category

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