Difference between revisions of "YMR221C"

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(Nitrogen Starvation)
(Community Commentary)
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*In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.
 
*In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.
  
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===[[UW-Stout/Bud Scars FA21]]===
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Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YBR184W cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells.
  
 
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Revision as of 14:18, 14 December 2021

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Systematic name YMR221C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XIII:715445..713931
Primary SGDID S000004834


Description of YMR221C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy[1][2]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Heat Shock FA21

Heat Shock.jpeg

YMR221C (C)

  • When stressing this strain of yeast, there was not much of a growth difference. The growth rate slowed down once heat shocked.

Nitrogen Starvation

YMR221C Nitrogen21.jpg

  • In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.

UW-Stout/Bud Scars FA21

Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YBR184W cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells.



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References

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  1. Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
  2. Tarassov K, et al. (2008) An in vivo map of the yeast protein interactome. Science 320(5882):1465-70 SGD PMID 18467557

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