YDL109C

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Systematic name YDL109C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr IV:267201..265258
Primary SGDID S000002267


Description of YDL109C: Putative lipase; involved in lipid metabolism; YDL109C is not an essential gene[1]




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Community Commentary

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UW-Stout/Heat Shock SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.

Results

YDL109C BY4735 Control YDL109C Graph BY4735 Control Graph

Interpretation

From the data gathered, there was a large positive effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were 350% more resistant to heat shock than the control.

UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene1.jpg


INTERPERTATION In the graph and photos above you can see that exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 90% of yeast cell cultures, in comparison to its control counterpart which was the the same gene and amount of cells, just was not exposed to UV Light.

UW-Stout/D2O SP22

4.28.2022 1.png

The YDL109C (A1) Knock Out Yeast Strain was a little more in the middle spectrum of results with being a little more resistant to the 35% dilution of D2O. The range it stays in as a maximum OD 600 is 0.3-0.4. With the growth curve decaying at the end at about 16 hours.

DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically higher expression in phosphorus limited chemostat cultures versus phosphorus excess. [2] [3]

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.





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References

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  1. Daum G, et al. (1999) Systematic analysis of yeast strains with possible defects in lipid metabolism. Yeast 15(7):601-14 SGD PMID 10341423
  2. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  3. submitted by Viktor Boer on 2003-07-25

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