YCL049C

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Systematic name YCL049C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr III:40724..39786
Primary SGDID S000000554


Description of YCL049C: Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene[1]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.


UW Stout/D2O SP22

4.28.2022 1.png

With YCL049C (A7) we see that this Knock-Out Yeast Strain is unaffected by the 35% dilution of D2O. You can see that the growth curve hits it limit at about 0.5-0.6 approximately.


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UW Stout/Sucrose Fermentation SP22

Gene Glucose Fructose Ethanol
Standard solution 2.0000 0.2000 2.0000
YDl109C 0.3800 0.3933 0.3430
YGL140C 0.2212 0.2685 0.1867
YOR111W 0.3332 0.3598 0.1343
YHL029C 0.3870 0.2368 0.1151
YDR307W 0.4366 0.2487 0.0606
YNL058C 0.2710 0.3056 0.1577
YCL049C
YGR079W
YBL113C 0.3498 0.2012 0.1434
BY4735 0.3171 0.3084 0.3541

UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene7.jpg


INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.

UW-Stout/Heat Shock SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.

Results

BY4735 Control YCL049C BY4735 Control Graph YCL049C Graph

Interpretation

From the data gathered, there was no effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were within the margin of error to be the same as the control cells.

References

See Help:References on how to add references

  1. Terashima H, et al. (2002) Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae. Curr Genet 40(5):311-6 SGD PMID 11935221

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