Difference between revisions of "YBR225W"

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The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Comparing the BY4735 and YBR225W doubling times in 750mM NaCl, there is a low to moderate effect on the growth rate. This effect positively influences the knockout strain's (YBR225W) growth.
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The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Comparing the YBR225W doubling times in 0mM and 750mM NaCl, there is little to no effect on the growth rate. This effect does nothing to influence the knockout strain's (YBR225W) growth.
 
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===[[UW-Stout/Caffeine_SP21|Caffeine Group 1]]===
 
===[[UW-Stout/Caffeine_SP21|Caffeine Group 1]]===

Revision as of 10:49, 27 April 2021

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Systematic name YBR225W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr II:670627..673329
Primary SGDID S000000429


Description of YBR225W: Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components[1][2]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

YBR225W-water.png

In a BY4735 background, knocking out YBR225W does not seem to have any effect on the strain's growth rate. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YBR225W knock-out strain's doubling time was 156 minutes. (These doubling times are the means of three experiments.)



Salt Concentration (NaCl)

NaCl-BY4735vYBR225W-Graphv2.JPG


0mM NaCl conc. BY4735 strain's doubling time: 169 minutes

0mM NaCl conc. YBR225W strain's doubling time: 224 minutes

750mM NaCl conc. BY4735 strain's doubling time: 294 minutes

750mM NaCl conc. YBR225W strain's doubling time: 226 minutes


The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Comparing the YBR225W doubling times in 0mM and 750mM NaCl, there is little to no effect on the growth rate. This effect does nothing to influence the knockout strain's (YBR225W) growth.


Caffeine Group 1

gene3vswildstrain.jpg


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References

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  1. Conde R, et al. (2003) Screening for new yeast mutants affected in mannosylphosphorylation of cell wall mannoproteins. Yeast 20(14):1189-211 SGD PMID 14587103
  2. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549

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