YCL049C
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| Systematic name | YCL049C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr III:40724..39786 |
| Primary SGDID | S000000554 |
Description of YCL049C: Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene[1]
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Contents
Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW Stout/D2O SP22
With YCL049C (A7) we see that this Knock-Out Yeast Strain is unaffected by the 35% dilution of D2O. You can see that the growth curve hits it limit at about 0.5-0.6 approximately.
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UW Stout/Sucrose Fermentation SP22
| Gene | Glucose | Fructose | Ethanol |
| Standard solution | 2.0000 | 0.2000 | 2.0000 |
| YDl109C | 0.3800 | 0.3933 | 0.3430 |
| YGL140C | 0.2212 | 0.2685 | 0.1867 |
| YOR111W | 0.3332 | 0.3598 | 0.1343 |
| YHL029C | 0.3870 | 0.2368 | 0.1151 |
| YDR307W | 0.4366 | 0.2487 | 0.0606 |
| YNL058C | 0.2710 | 0.3056 | 0.1577 |
| YCL049C | 0.4078 | 0.3052 | 0.1969 |
| YGR079W | 0.4042 | 0.1589 | 0.0080 |
| YBL113C | 0.3498 | 0.2012 | 0.1434 |
| BY4735 | 0.3171 | 0.3084 | 0.3541 |
Interpretation
This gene produced 55.61% of the amount of ethanol that the wild type produced.
UW-Stout/UV Light SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.
RESULTS
INTERPERTATION
In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
UW-Stout/Heat Shock SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.
Results
Interpretation
From the data gathered, there was no effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were within the margin of error to be the same as the control cells.
UW-Stout/Caffeine SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 4mM of caffeine.
Results
- BY4735(wild type yeast) and YCL049C(knockout yeast gene) growth after being subjected to 4mM caffeine solution.
Interpretation
UW-Stout/Hydrogen Peroxide SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to hydrogen peroxide.
Results
BY4735 and YCL049C after being exposed to 0.065% dilution of hydrogen peroxide solution.
Interpretation
From the growth curve, it can be observed that while the sample of the knockout strain was inhibited when treated with hydrogen peroxide, data range was very close to the control. This suggests that YCL049C may simply grow slower than wild type cells.
References
See Help:References on how to add references
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
UW-Stout/Nonionic Detergent SP22
Interpretations:
-According to the graph above, we can see that the KO yeast cell YCL049C follow a smaller sensitivity to the stress than the Wild type strain.
-We can then conclude that the knocked-out gene in YCL049C doesn't have a significant impact on the growth of the yeast cell.
