YHL012W
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| Systematic name | YHL012W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VIII:78936..80417 |
| Primary SGDID | S000001004 |
Description of YHL012W: Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase[1]
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Contents
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Heat Shock FA21
YHLO12W (B)
- When stressing out this strain of yeast, it had no growth. On the control plate, there was normal growth. So we can see that this yeast strain is sensitive to heat.
Nitrogen Starvation
- There was no significant change between the plates with NH₄ and without. They seemed to be growing at the same pace. So this particular gene was not affected by the Nitrogen Starvation stress.
UW-Stout/Bud Scars FA21
Cells grown with this gene (YHL012W) knocked out had an average of 1.20 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 106 YHL012W cells counted, 127 bud scars were seen. This indicated that the YHL012W gene slows reproduction rate and thus bud scarring in yeast cells.
pH
YHL012W
- When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain
Protocol: [[1]]
UW-Stout/Ethanol FA21
- The Yeast Strain of YHL012W was the one that was the most affected by the 18.5 % Ethanol it still has a steady growth curve
UW-Stout/G418 FA21
The YHL012W yeast strain doubling time without any stress was 187 minutes which is very similar to the wild type with 182 minutes. When both strains were stressed by G418 they YHL012W doubling time was 209 minutes and the wild type was 178 minutes. (These times are averaged between three trials) Protocol: [2]
UW-Stout/Copper FA21
- In these images you can see the growth of the yeast. In the right image this is a normal growth of yeast and in the left one we can see the yeast clumped up in the center of the yeast cell. This is strange because the image in the left is showing the yeast floating on top of the Copper Sulfate solution and it is collected in the center of the well which is different from the normal yeast which is evenly distributed in the well. The yeast was different was the YHL012W gene was removed so this indicates that the yeast reacted differently to this gene when stressed versus the other genes.
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References
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