YHL012W
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| Systematic name | YHL012W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VIII:78936..80417 |
| Primary SGDID | S000001004 |
Description of YHL012W: Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase[1]
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Contents
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Heat Shock FA21
YHLO12W (B)
- When stressing out this strain of yeast, it had no growth. On the control plate, there was normal growth. So we can see that this yeast strain is sensitive to heat.
Nitrogen Starvation
- There was no significant change between the plates with NH₄ and without. They seemed to be growing at the same pace. So this particular gene was not affected by the Nitrogen Starvation stress.
UW-Stout/Bud Scars FA21
Cells grown with this gene (YHL012W) knocked out had an average of 1.20 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 106 YHL012W cells counted, 127 bud scars were seen. This indicated that the YHL012W gene slows reproduction rate and thus bud scarring in yeast cells.
pH
YHL012W
- When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain
Protocol: [[1]]
UW-Stout/G418 FA21
[File:YHL012W Vs BY4735 G418.png|left|thumb|400px]]
The YHL012W yeast strain doubling time without any stress was 187 minutes which is very similar to the wild type with 182 minutes. When both strains were stressed by G418 they YHL012W doubling time was 209 minutes and the wild type was 178 minutes. (These times are averaged between three trials) Protocol: [2]
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