UW-Stout/About

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About the UW-Stout Orphan Gene Project

Starting in the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout has been knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.

For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.

Knock-out strains (Spring 2019)

YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C

Protocols (Spring 2019)

Basic growth curve protocol

Heat Shock

G-418 Stress

Nitrogen Starvation

Fermentation

Methanol

Caffeine

pH Stress