Difference between revisions of "YDR307W"

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(Community Commentary)
(UW Stout/Sucrose Fermentation SP22)
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====Interpretation====
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This gene produced 17.11% of the amount of ethanol that the wild type produced. It is possible that this gene plays a role in fermentation because of such a low amount of ethanol production.
  
 
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Revision as of 12:35, 3 May 2022

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Systematic name YDR307W
Gene name PMT7
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr IV:1075865..1077853
Primary SGDID S000002715


Description of YDR307W: Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation[1]




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Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.



UW Stout/D2O SP22

4.28.2022 1.png

The YDR307W Knock Out Yeast Strain is A5 and is fairly unaffected by the 35% dilution of D2O, however, has a slight sensitivity to the dilution. With the maximum value ranging along the lines of 0.4-0.5 OD 600.

UW Stout/Sucrose Fermentation SP22

Gene Glucose Fructose Ethanol
Standard solution 2.0000 0.2000 2.0000
YDl109C 0.3800 0.3933 0.3430
YGL140C 0.2212 0.2685 0.1867
YOR111W 0.3332 0.3598 0.1343
YHL029C 0.3870 0.2368 0.1151
YDR307W 0.4366 0.2487 0.0606
YNL058C 0.2710 0.3056 0.1577
YCL049C
YGR079W
YBL113C 0.3498 0.2012 0.1434
BY4735 0.3171 0.3084 0.3541

Interpretation

This gene produced 17.11% of the amount of ethanol that the wild type produced. It is possible that this gene plays a role in fermentation because of such a low amount of ethanol production.

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UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene5.jpg


INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 80% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.

References

See Help:References on how to add references

  1. Gentzsch M and Tanner W (1996) The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital. EMBO J 15(21):5752-9 SGD PMID 8918452

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