Difference between revisions of "UW-Stout/About"

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[[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a  
 
[[UW-Stout/Knockout_Protocol|knocking out]] [https://yeastmine.yeastgenome.org/yeastmine/bagDetails.do?scope=all&bagName=Uncharacterized_ORFs genes of unknown function] in a  
 
[https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]
 
[https://www.atcc.org/en/Products/All/200897.aspx BY4735 strain]
background, then screening those knockout strains for phenotypes.  This page organizes our methods, data and conclusions from this project.
+
background, then screening those knockout strains for phenotypes.  This page organizes the methods and data from this project.
  
 
For more information, or to request a knock-out strain, please contact Dr. Brian Teague  
 
For more information, or to request a knock-out strain, please contact Dr. Brian Teague  

Revision as of 19:30, 29 April 2019

About the UW-Stout Orphan Gene Project

Beginning in the spring of 2019, the Intro Cell and Molecular Biology class at the University of Wisconsin -- Stout began knocking out genes of unknown function in a BY4735 strain background, then screening those knockout strains for phenotypes. This page organizes the methods and data from this project.

For more information, or to request a knock-out strain, please contact Dr. Brian Teague at teagueb@uwstout.edu.

Knock-out strains (Spring 2019)

YCL002C YCR100C YEL035C YFL064C YGL235W YJL043W YMR090W YPL068C

Protocols (Spring 2019)

Basic growth curve protocol

Heat Shock

G-418 Stress

Nitrogen Starvation

Fermentation

Methanol

Caffeine

pH Stress