Difference between revisions of "YCR100C"

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{{UW-Stout}}
 
{{UW-Stout}}
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===[[UW-Stout/Sensitivity To Nitrogen Starvation]]===
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[[File: YCR100C_growth plate.JPG|thumb|Growth of YCR100C on synthetic defined media (SDC).]]
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[[File: YCR100C_starvation plate.JPG|thumb|Growth of YCR100C on omission media that lacks nitrogen.]]
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[[File: YCR100C_revival plate.JPG|thumb|Growth of transferred BY4735 on Synthetic defined media.]]
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Knocking out the YCR100C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
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===[[UW-Stout/Growth_Curve|Growth Curve]]===
 
===[[UW-Stout/Growth_Curve|Growth Curve]]===
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YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.  
 
YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.  
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===[[UW-Stout/G-418 | G-418 Stress]]===
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[[File:image033.png]]
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In the BY4735 background, knocking out the YCR100C strain seem to have a very small effect on a growth rate by slowing it down. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YCR100C knock-out strain's doubling time was 235 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YCR100C knock-out strain's doubling time was 92 minutes.
  
 
===Methanol Sensitivity===
 
===Methanol Sensitivity===
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The wild type had a doubling time of 150 min, the YCR100c had a doubling time of 350 min. The methanol slowed the yeast down and that means this strain is more sensitive.
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[https://wiki.yeastgenome.org/index.php/UW-Stout/Methanol]
  
 
[[File:YCR100R_methanol_sensitivity.png]]
 
[[File:YCR100R_methanol_sensitivity.png]]
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===Heat Shock===
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[[File:YCR100C Heat Shock.PNG]]
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Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.
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===Stressing with Hydroxyurea===
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[[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]]
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-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCR100C showed about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.
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[[file:HU_YCR100C.png|left]]
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===Fermentation===
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Fermentation protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]
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- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
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[[File:YCR100C.PNG]]
  
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 
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J Biol Chem 278(5):3265-74</ref>
 
J Biol Chem 278(5):3265-74</ref>
 
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===Caffeine Sensitivity===
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[[File:YCR100C.png|YCR100C VS BY4735]] CAffeine PRotocol [[UW-Stout/Caffeine]] YCR100C and the Wild Type yeast had a very similar reaction to the caffeine.
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----
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===Stressing with pH===
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pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
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- The wild type strain had a significant difference in doubling time when my partner and I stressed it out with a varying levels of acidic pH's and It displayed that when we tested it on the final lab experiment of a pH of 4 that it was highly sensitive to that particular pH and In some of the test conditions that it displayed that they have died or are very very slowly growing. But the yeast gene strain YCL002C we came to the conclusion that it was a lot more resilient to the acidic pH of 4 than the wild type strain.
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[[File:----
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===Stressing with pH===
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pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
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When we looked at the line graph we saw that the data for each strain were pretty close to each other but there was still a growth difference when we compared the yeast gene strain YCR100C to the wild type strain BY4735 but not that much to say that either one of them is more resilient than the other. So, Therefore our conclusion is that each strain is both resilient to the acidic pH of 4.
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[[File:pH-graph-YCR100C.PNG]]
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===Cold Shock Sensitivity===
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[[File:YCR100CBB.png]]
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Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
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YCR100C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YCR100C.
  
 
==References==
 
==References==
 
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Latest revision as of 19:07, 9 May 2019

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Systematic name YCR100C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr III:302221..301271
Primary SGDID S000000697


Description of YCR100C: Putative protein of unknown function[1]




Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YCR100C on synthetic defined media (SDC).
Growth of YCR100C on omission media that lacks nitrogen.
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YCR100C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.


Growth Curve

ycr100c growth.png

In a BY4735 background, knocking out YCR100C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCR100C knock-out strain's doubling time was 219 minutes.

Ultraviolet Sensitivity

YCR100C uv light.jpg BY4735(wild) uv light.jpg

YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

G-418 Stress

image033.png

In the BY4735 background, knocking out the YCR100C strain seem to have a very small effect on a growth rate by slowing it down. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YCR100C knock-out strain's doubling time was 235 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YCR100C knock-out strain's doubling time was 92 minutes.

Methanol Sensitivity

The wild type had a doubling time of 150 min, the YCR100c had a doubling time of 350 min. The methanol slowed the yeast down and that means this strain is more sensitive.

[1]

YCR100R methanol sensitivity.png


Heat Shock

YCR100C Heat Shock.PNG

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCR100C showed about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.

HU YCR100C.png



Fermentation

Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

YCR100C.PNG



Caffeine Sensitivity

YCR100C VS BY4735 CAffeine PRotocol UW-Stout/Caffeine YCR100C and the Wild Type yeast had a very similar reaction to the caffeine.

Stressing with pH

pH stress protocol link [3]


- The wild type strain had a significant difference in doubling time when my partner and I stressed it out with a varying levels of acidic pH's and It displayed that when we tested it on the final lab experiment of a pH of 4 that it was highly sensitive to that particular pH and In some of the test conditions that it displayed that they have died or are very very slowly growing. But the yeast gene strain YCL002C we came to the conclusion that it was a lot more resilient to the acidic pH of 4 than the wild type strain.


[[File:----

Stressing with pH

pH stress protocol link [4]


When we looked at the line graph we saw that the data for each strain were pretty close to each other but there was still a growth difference when we compared the yeast gene strain YCR100C to the wild type strain BY4735 but not that much to say that either one of them is more resilient than the other. So, Therefore our conclusion is that each strain is both resilient to the acidic pH of 4.


pH-graph-YCR100C.PNG

Cold Shock Sensitivity

YCR100CBB.png


Protocol: Cold Shock


YCR100C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YCR100C.

References

See Help:References on how to add references

  1. ↑ SGD (2006) No information available SGD PMID

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