Difference between revisions of "YGL235W"

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In a BY4735 background, knocking out YGL235W seems to have a moderate effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 124 minutes, while the YGL235W knock-out strain's doubling time was 209 minutes.  
 
In a BY4735 background, knocking out YGL235W seems to have a moderate effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 124 minutes, while the YGL235W knock-out strain's doubling time was 209 minutes.  
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===[[UW-Stout/G-418 | G-418 Stress]]===
  
 
[[File:image003.png]]
 
[[File:image003.png]]
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In the BY4735 background, knocking out YGL235W seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YGL235W knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YGL235W knock-out strain's doubling time was 93 minutes.
  
 
===Methanol Sensitivity===
 
===Methanol Sensitivity===
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[[file:YGL235W_Methanol_Sensitivity.png]]
 
[[file:YGL235W_Methanol_Sensitivity.png]]
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===Heat Shock===
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[[File:YGL235W Heat Shock.PNG]]
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Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.
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===Stressing with Hydroxyurea===
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[[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]]
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-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YGL235W had about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.
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[[file:HU_YGL235W.png|left]]
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===Fermentation===
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Fermentation protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]
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- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
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[[File:fer YGL235W.PNG]]
  
 
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J Biol Chem 278(5):3265-74</ref>
 
J Biol Chem 278(5):3265-74</ref>
 
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===Caffeine Sensitivity===
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[[File:YGL235W_vs_BY4735.jpg|YGL235W VS BY4735]]
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Caffeine Protocol [[UW-Stout/Caffeine]]
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Compared to the wild type yeast strain this gene was more sensitive to caffeine.
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----
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===Stressing with pH===
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pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
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The yeast strain YGL235W had somewhat of a significant difference compared to the wild type even though the wild type keeps growing but not as fast as the yeast strain YG1235W does and the strain does plato's at the end and continues to plummets and the wild type keeps slowly climbing at a steady rate of growth and time. So, Therefore we have concluded that the data signifies that the test condition was inconclusive to the experiment.
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[[File:pH-YGL235W.PNG]]
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===Cold Shock Sensitivity===
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[[File:YGL235WBB.png]]
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Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
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YGL235W is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YGL235W.
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==References==
 
==References==
 
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Latest revision as of 18:53, 17 December 2019

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Systematic name YGL235W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VII:55279..55815
Primary SGDID S000003204


Description of YGL235W: Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2[1][2]




Community Commentary

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Protein Details

Protein Modification

Modification(s): Phosphorylation

Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [2] [3]

UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YGL235W on synthetic defined media (SDC).
Growth of YFL064C on omission media that lacks nitrogen.
File:YGL235W revival plate.JPG
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YGL235Wseems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.


Ultraviolet Sensitivity

YGL235W uv light.jpg BY4735(wild) uv light.jpg

YGL235W is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

ygl235w growth.png

In a BY4735 background, knocking out YGL235W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YGL235W knock-out strain's doubling time was 209 minutes.

G-418 Stress

image003.png

In the BY4735 background, knocking out YGL235W seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YGL235W knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YGL235W knock-out strain's doubling time was 93 minutes.

Methanol Sensitivity

The wild type had a doubling time of 160 min, the YGL235w had a doubling time of 160 min. The strain was sensitive to the methanol.

[1]

YGL235W Methanol Sensitivity.png


Heat Shock

YGL235W Heat Shock.PNG

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YGL235W had about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.

HU YGL235W.png

Fermentation

Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

fer YGL235W.PNG


Caffeine Sensitivity

YGL235W VS BY4735 Caffeine Protocol UW-Stout/Caffeine Compared to the wild type yeast strain this gene was more sensitive to caffeine.

Stressing with pH

pH stress protocol link [3]


The yeast strain YGL235W had somewhat of a significant difference compared to the wild type even though the wild type keeps growing but not as fast as the yeast strain YG1235W does and the strain does plato's at the end and continues to plummets and the wild type keeps slowly climbing at a steady rate of growth and time. So, Therefore we have concluded that the data signifies that the test condition was inconclusive to the experiment.

pH-YGL235W.PNG

Cold Shock Sensitivity

YGL235WBB.png


Protocol: Cold Shock


YGL235W is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YGL235W.


References

See Help:References on how to add references

  1. ↑ Chamilos G, et al. (2008) Genomewide Screening for Genes Associated with Gliotoxin Resistance and Sensitivity in Saccharomyces cerevisiae. Antimicrob Agents Chemother 52(4):1325-9 SGD PMID 18212113
  2. ↑ 2.0 2.1 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64 SGD PMID 14574415 Cite error: Invalid <ref> tag; name "S000074306" defined multiple times with different content
  3. ↑ submitted by Jeff Ubersax on 2004-01-27

See Help:Categories on how to add the wiki page for this gene to a Category

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