Difference between revisions of "YPL068C"

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YPL068C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.  
 
YPL068C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.  
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===[[UW-Stout/G-418 | G-418 Stress]]===
  
 
[[File:image008.png]]
 
[[File:image008.png]]
  
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In the BY4735 background, knocking out YPL068C seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YPL068C knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YPL068C knock-out strain's doubling time was 92 minutes.
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===[[UW-Stout/Heat Shock|Heat Shock]]===
 
[[File:HeatShockforYP.png]]
 
[[File:HeatShockforYP.png]]
  
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<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
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Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.
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Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
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===Methanol Sensitivity===
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<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.  
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J Biol Chem 278(5):3265-74</ref>
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The wild type had a doubling time of 150 min, the YPL068c had a doubling time of 200 min. The doubling time increased which means this strain is sensitive to methanol.
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[https://wiki.yeastgenome.org/index.php/UW-Stout/Methanol]
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[[file:YPL068C_Methanol_sensitivity.png]]
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===Stressing with Hydroxyurea===
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[[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]]
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[[file:HU_YPL068C.png]]
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The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YPL068C showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays some role in DNA replication.
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===Fermentation===
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Fermentation protocol link [[https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]]
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There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
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[[File:Fer YPL068C.PNG]]
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===Caffeine Sensitivity===
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[[File:YPL068C.jpg|YPL068C VS BY4735]]
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Caffeine Protocol [[UW-Stout/Caffeine], the genes reacted the same at the beginning, varied in the middle then came back together at the end.
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===Stressing with pH===
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pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
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When I looked at the data this graph shows I see that both the Wild type and also the yeast strain YPL068C have about the same linear pathway and so I came to the conclusion that even though the yeast strain YPL068C has somewhat a deviation difference but its still small enough and somewhat close enough to the wild types and so therefore both of them are yet resilient to the pH 4 stress test.
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[[File:pH-YPL068.PNG]]
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===Cold Shock Sensitivity===
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[[File:YPL068CBB.png]]
  
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Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
  
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YPL068C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YPL068C.
  
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

Latest revision as of 11:49, 31 May 2019

Share your knowledge...Edit this entry!

Systematic name YPL068C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XVI:425096..424215
Primary SGDID S000005989


Description of YPL068C: Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS[1][2]




Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

ypl068 growth.png

In a BY4735 background, knocking out YPL068C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YPL068C knock-out strain's doubling time was 209 minutes.

Ultraviolet Sensitivity

YPL068C UV light.jpeg BY4735(wild) uv light.jpg

YPL068C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.


G-418 Stress

image008.png

In the BY4735 background, knocking out YPL068C seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YPL068C knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YPL068C knock-out strain's doubling time was 92 minutes.

Heat Shock

HeatShockforYP.png

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Methanol Sensitivity

The wild type had a doubling time of 150 min, the YPL068c had a doubling time of 200 min. The doubling time increased which means this strain is sensitive to methanol.

[1]

YPL068C Methanol sensitivity.png

Stressing with Hydroxyurea

Hydroxyurea Protocol

HU YPL068C.png


The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YPL068C showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays some role in DNA replication.


Fermentation

Fermentation protocol link [[2]]

There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Fer YPL068C.PNG


Caffeine Sensitivity

YPL068C VS BY4735

Caffeine Protocol [[UW-Stout/Caffeine], the genes reacted the same at the beginning, varied in the middle then came back together at the end.


Stressing with pH

pH stress protocol link [3]


When I looked at the data this graph shows I see that both the Wild type and also the yeast strain YPL068C have about the same linear pathway and so I came to the conclusion that even though the yeast strain YPL068C has somewhat a deviation difference but its still small enough and somewhat close enough to the wild types and so therefore both of them are yet resilient to the pH 4 stress test.

pH-YPL068.PNG

Cold Shock Sensitivity

YPL068CBB.png

Protocol: Cold Shock

YPL068C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YPL068C.


References

See Help:References on how to add references

  1. ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  2. ↑ Lee MW, et al. (2007) Global protein expression profiling of budding yeast in response to DNA damage. Yeast 24(3):145-54 SGD PMID 17351896

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