YCL002C

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Systematic name	YCL002C
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr III:111675..110808
Primary SGDID	S000000508

Description of YCL002C: Putative protein of unknown function; YCL002C is not an essential gene

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UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YCLO02C on synthetic defined media (SDC).

Growth of YCLO02C on omission media that lacks nitrogen.

Knocking out the YCLO02C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YCL002C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Rate in YPD

In a BY4735 background, knocking out YCL002C seems to have no effect on growth rate in log-phase in YPD. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCL002C knock-out strain's doubling time was 134 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.

G-418 Stress

In the BY4735 background, knocking out YCL002C seems to have a little effect on the growth rate by slowing it down. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YCL002C knock-out strain's doubling time was 172 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YCL002C knock-out strain's doubling time was 66 minutes.

Methanol Sensitivity

The wild type had a doubling time of 175 minutes, the YCL002 had a doubling time of 315 minutes. The addition of methanol sped up our reaction and caused the yeast cells to multiply faster.

[1]

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCL002C also portrayed a small difference in doubling time, indicating that this gene most likely does not play a role in DNA replication.

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Stressing with pH

pH stress protocol link [2]

- The wild type strain had a significant difference in doubling time when my partner and I stressed it out with a varying levels of acidic pH's and It displayed that when we tested it on the final lab experiment of a pH of 4 that it was highly sensitive to that particular pH and In some of the test conditions that it displayed that they have died or are very very slowly growing. But the yeast gene strain YCL002C we came to the conclusion that it was a lot more resilient to the acidic pH of 4 than the wild type strain.

Transient Heat Shock

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Fermentation

Fermentation protocol link [3] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Caffeine Sensitivity

Caffeine experiment protocol UW-Stout/Caffeine

It appears the caffeine concentration had a very similar effect on this gene and the wild type yeast cells.

Cold Shock Sensitivity

Protocol: Cold Shock

YCL002C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YCL002C.

References

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