Difference between revisions of "YCL002C"

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==Community Commentary==
 
==Community Commentary==
 
{{CommentaryHelp}}
 
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===[[UW-Stout/Sensitivity To Nitrogen Starvation]]===
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[[File: YCLO02C_growth plate.JPG|thumb|Growth of YCLO02C on synthetic defined media (SDC).]]
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[[File: YCL002C starvation plate1.JPG|thumb|Growth of YCLO02C on omission media that lacks nitrogen.]]
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Knocking out the YCLO02C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
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===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===
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[[File:YCL002C uv light.jpg.jpg|300 px]]
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[[File:BY4735(wild) uv light.jpg|300 px]]
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YCL002C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
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In a BY4735 background, knocking out YCL002C seems to have no effect on growth rate in log-phase in YPD.  In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCL002C knock-out strain's doubling time was 134 minutes.  However, the knock-out strain did reach a substantially higher OD600 before saturating.
 
In a BY4735 background, knocking out YCL002C seems to have no effect on growth rate in log-phase in YPD.  In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCL002C knock-out strain's doubling time was 134 minutes.  However, the knock-out strain did reach a substantially higher OD600 before saturating.
  
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[[File:image001.png]]
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===[[UW-Stout/G-418 | G-418 Stress]]===
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[[File:image017.png]]
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In the BY4735 background, knocking out YCL002C seems to have a little effect on the growth rate by slowing it down.  In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YCL002C knock-out strain's doubling time was 172 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YCL002C knock-out strain's doubling time was 66 minutes.
  
 
===Methanol Sensitivity===
 
===Methanol Sensitivity===
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[[File:YCL002C_Methanol.png]]  
 
[[File:YCL002C_Methanol.png]]  
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===Stressing with Hydroxyurea===
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[[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]]
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-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCL002C also portrayed a small difference in doubling time, indicating that this gene most likely does not play a role in DNA replication.
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[[file:HU_YCL002C.png|left]]
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----
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===Stressing with pH===
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pH stress protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/pH]
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- The wild type strain had a significant difference in doubling time when my partner and I stressed it out with a varying levels of acidic pH's and It displayed that when we tested it on the final lab experiment of a pH of 4 that it was highly sensitive to that particular pH and In some of the test conditions that it displayed that they have died or are very very slowly growing. But the yeast gene strain YCL002C we came to the conclusion that it was a lot more resilient to the acidic pH of 4 than the wild type strain.
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[[File:Examples.PNG]]
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===Transient Heat Shock===
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[[File:YCL002C Heat Shock.PNG]]
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Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.
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===Fermentation===
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Fermentation protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]
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- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
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[[File:Fer YGL002C.PNG]]
  
 
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J Biol Chem 278(5):3265-74</ref>
 
J Biol Chem 278(5):3265-74</ref>
 
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===Caffeine Sensitivity===
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[[File:YCL002C_vs_BY4735.jpg|YCL002C VS BY4735]]
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Caffeine experiment protocol [[UW-Stout/Caffeine]]
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It appears the caffeine concentration had a very similar effect on this gene and the wild type yeast cells.
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===Cold Shock Sensitivity===
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[[File:YCL002C.png]]
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Protocol: [https://wiki.yeastgenome.org/index.php/UW-Stout/Cold_Shock Cold Shock]
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YCL002C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YCL002C.
  
 
==References==
 
==References==
 
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Latest revision as of 18:37, 9 May 2019

Share your knowledge...Edit this entry!

Systematic name YCL002C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr III:111675..110808
Primary SGDID S000000508


Description of YCL002C: Putative protein of unknown function; YCL002C is not an essential gene[1]




Community Commentary

About Community Commentary. Please share your knowledge!


UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YCLO02C on synthetic defined media (SDC).
Growth of YCLO02C on omission media that lacks nitrogen.

Knocking out the YCLO02C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YCL002C uv light.jpg.jpg BY4735(wild) uv light.jpg

YCL002C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.


This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Rate in YPD

ycl002c growth.png

In a BY4735 background, knocking out YCL002C seems to have no effect on growth rate in log-phase in YPD. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCL002C knock-out strain's doubling time was 134 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.

G-418 Stress

image017.png

In the BY4735 background, knocking out YCL002C seems to have a little effect on the growth rate by slowing it down. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YCL002C knock-out strain's doubling time was 172 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YCL002C knock-out strain's doubling time was 66 minutes.

Methanol Sensitivity

The wild type had a doubling time of 175 minutes, the YCL002 had a doubling time of 315 minutes. The addition of methanol sped up our reaction and caused the yeast cells to multiply faster.

[1]

YCL002C Methanol.png

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCL002C also portrayed a small difference in doubling time, indicating that this gene most likely does not play a role in DNA replication.

HU YCL002C.png


Stressing with pH

pH stress protocol link [2]


- The wild type strain had a significant difference in doubling time when my partner and I stressed it out with a varying levels of acidic pH's and It displayed that when we tested it on the final lab experiment of a pH of 4 that it was highly sensitive to that particular pH and In some of the test conditions that it displayed that they have died or are very very slowly growing. But the yeast gene strain YCL002C we came to the conclusion that it was a lot more resilient to the acidic pH of 4 than the wild type strain.


Examples.PNG

Transient Heat Shock

YCL002C Heat Shock.PNG

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Fermentation

Fermentation protocol link [3] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Fer YGL002C.PNG


Caffeine Sensitivity

YCL002C VS BY4735

Caffeine experiment protocol UW-Stout/Caffeine

It appears the caffeine concentration had a very similar effect on this gene and the wild type yeast cells.

Cold Shock Sensitivity

YCL002C.png


Protocol: Cold Shock


YCL002C is neither more or less sensitive to the cold than the wild type strain. This is shown because the doubling times change the same amount before and after the cold shock in both the wild type strain and YCL002C.

References

See Help:References on how to add references

  1. ↑ Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549

See Help:Categories on how to add the wiki page for this gene to a Category

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