Difference between revisions of "YOL066C"

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'''Description of YOL066C:''' DRAP deaminase, catalyzes the third step of the riboflavin biosynthesis pathway; cytoplasmic tRNA pseudouridine synthase involved in pseudouridylation of cytoplasmic tRNAs at position 32<ref name='S000054618'>Richter G, et al. (1997) Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis. J Bacteriol 179(6):2022-8 {{SGDpaper|S000054618}} PMID 9068650</ref><ref name='S000080014'>Behm-Ansmant I, et al. (2004) Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae. J Biol Chem 279(51):52998-3006 {{SGDpaper|S000080014}} PMID 15466869</ref><ref name='S000072829'>Urban A, et al. (2003) Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae. J Chromatogr B Analyt Technol Biomed Life Sci 786(1-2):187-95
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'''Description of YOL066C:''' Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs<ref name='S000054618'>Richter G, et al. (1997) Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis. J Bacteriol 179(6):2022-8 {{SGDpaper|S000054618}} PMID 9068650</ref><ref name='S000080014'>Behm-Ansmant I, et al. (2004) Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae. J Biol Chem 279(51):52998-3006 {{SGDpaper|S000080014}} PMID 15466869</ref><ref name='S000072829'>Urban A, et al. (2003) Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae. J Chromatogr B Analyt Technol Biomed Life Sci 786(1-2):187-95
 
  {{SGDpaper|S000072829}} PMID 12651014</ref>
 
  {{SGDpaper|S000072829}} PMID 12651014</ref>
 
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Revision as of 14:05, 21 May 2009

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Systematic name YOL066C
Gene name RIB2
Aliases PUS8
Feature type ORF, Verified
Coordinates Chr XV:204471..202696
Primary SGDID S000005427


Description of YOL066C: Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs[1][2][3]




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References

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  1. Richter G, et al. (1997) Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis. J Bacteriol 179(6):2022-8 SGD PMID 9068650
  2. Behm-Ansmant I, et al. (2004) Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae. J Biol Chem 279(51):52998-3006 SGD PMID 15466869
  3. Urban A, et al. (2003) Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae. J Chromatogr B Analyt Technol Biomed Life Sci 786(1-2):187-95 SGD PMID 12651014

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