YOL066C

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Systematic name YOL066C
Gene name RIB2
Aliases PUS8
Feature type ORF, Verified
Coordinates Chr XV:204471..202696
Primary SGDID S000005427


Description of YOL066C: Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs[1][2][3][4]




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References

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  1. Behm-Ansmant I, et al. (2004) Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae. J Biol Chem 279(51):52998-3006 SGD PMID 15466869
  2. Oltmanns O and Bacher A (1972) Biosynthesis of riboflavine in Saccharomyces cerevisiae: the role of genes rib 1 and rib 7. J Bacteriol 110(3):818-22 SGD PMID 4555411
  3. Richter G, et al. (1997) Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis. J Bacteriol 179(6):2022-8 SGD PMID 9068650
  4. Urban A, et al. (2003) Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae. J Chromatogr B Analyt Technol Biomed Life Sci 786(1-2):187-95 SGD PMID 12651014

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