Difference between revisions of "YEL013W"

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=== Interactions ===
 
[[Category:Topic:Interactions]]
 
==== Physical ====
 
[[Category:Topic:Interactions:Physical]]
 
Physical interaction with VAC17 [[Category:Physical Interactions with VAC17]]<br>
 
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 
 
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==== Two Hybrid ====
 
[[Category:Topic:Interactions:Two Hybrid]]
 
Two Hybrid interaction with VAC17 [[Category:Two Hybrid Interactions with VAC17]]<br>
 
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 
 
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==Community Commentary==
 
==Community Commentary==

Revision as of 11:51, 24 January 2007

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Systematic name YEL013W
Gene name VAC8
Aliases YEB3
Feature type ORF, Verified
Coordinates Chr V:128825..130561
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Description of YEL013W: Phosphorylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions[1][2][3]



Community Commentary

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Interactions

Physical

Physical interaction with VAC17
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [4] [5]


Two Hybrid

Two Hybrid interaction with VAC17
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [4] [5]


References

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  1. Wang YX, et al. (1998) Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. J Cell Biol 140(5):1063-74 SGD PMID 9490720
  2. Scott SV, et al. (2000) Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting. J Biol Chem 275(33):25840-9 SGD PMID 10837477
  3. Pan X, et al. (2000) Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p. Mol Biol Cell 11(7):2445-57 SGD PMID 10888680
  4. 4.0 4.1 Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 SGD PMID 12594460
  5. 5.0 5.1 submitted by Fusheng Tang on 2003-03-17

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