YDR477W

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Systematic name YDR477W
Gene name SNF1
Aliases CAT1, CCR1, GLC2, HAF3, PAS14
Feature type ORF, Verified
Coordinates Chr IV:1412373..1414274
Primary SGDID S000002885


Description of YDR477W: AMP-activated serine/threonine protein kinase found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis[1][2]




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Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b
Genotype: MCY4461
Together with: REG1
Phenotype(s): Loss of function (Null)

Deletion of SNF1 suppresses the hyperinvasive phenotype of reg1. [3] [4]


Interactions

Genetic

YDR477W is suppressed by Nrg2


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [3] [5]


YDR477W is suppressed by Nrg1


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg1 partially suppresses Snf1's defect in invasive growth, FLO11 expression, and STA2 expression. [3] [5]


Physical

Physical interaction with Nrg2
Snf1 coimmunoprecipitates with HA3-Nrg2 [6] [5]


Physical interaction with Nrg2
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [6] [5]


Physical interaction with Nrg1
Snf1 coimmunoprecipitates with HA3-Nrg1 [6] [5]


Physical interaction with Nrg1
LexA-Snf1 coimmunoprecipitates HA3-Nrg1 [6] [5]


Two Hybrid

Two Hybrid interaction with Nrg2
Snf1 kinase domain interacts with Nrg2 [6] [5]


Two Hybrid interaction with nrg1
Snf1 kinase domain interacts with Nrg1 [6] [5]





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References

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  1. Hardie DG, et al. (1998) The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell? Annu Rev Biochem 67():821-55 SGD PMID 9759505
  2. Sanz P (2003) Snf1 protein kinase: a key player in the response to cellular stress in yeast. Biochem Soc Trans 31(Pt 1):178-81 SGD PMID 12546680
  3. 3.0 3.1 3.2 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
  4. submitted by Valmik K. Vyas on 2003-03-27
  5. 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 submitted by Valmik K. Vyas on 2003-02-04
  6. 6.0 6.1 6.2 6.3 6.4 6.5 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 SGD PMID 11404322

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