Difference between revisions of "YMR144W"
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====Protocol==== | ====Protocol==== | ||
| − | [ | + | [https://wiki.yeastgenome.org/index.php/UW-Stout/G418_SP23] |
==Glucose Sensitivity== | ==Glucose Sensitivity== | ||
Revision as of 14:48, 5 May 2023
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| Systematic name | YMR144W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XIII:553362..554390 |
| Primary SGDID | S000004752 |
Description of YMR144W: Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene[1][2]
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Contents
Acids
Analysis
These are the results we gathered from testing a pH of # against the control wild yeast and the gene YMR144W. The three red lines are the growth rates of the BY (wild yeast) mixed with a pH of # and the three blue lines are the three growth rates of the YMR144W knockout gene mixed with a pH of #. All of the trials showed great growth rates. The average double time of the wild yeast was 13.57 minutes and the average double time of the YMR144W gene was 10.17 minutes. The YMR144W growth rates did grow at a slower rate compared to the wild yeast. All trials did demonstrate strong cell growth over the 18 hours.
Caffeine
Analysis
Isopropanol
Analysis
These results were not consistent as there are 3 very different results. The first trial was way off what it was supposed to be and therefore could have been contaminated or been an error in the pipetting process. The second trial was the most successful and gave the results that were expected. The third trial was good except for the region from about 8:00-11:20 where it spiked up and down. The last 2 had more growth than the control which could mean that the cell without the gene that was knocked out is able to withstand more alcohol.
Geneticin
Protocol
Glucose Sensitivity
Protocol
Analysis
The graph displayed above shows a viability assay comparison with YMR144W knockout strain to BY4735 (wild type) and others. Results are shown as amount of dead cells per 100 assayed via hemocytometer. We found from our data that there was approximately a 15% decrease in the cell population. It was surprising to find that by removing this gene there was an increase in cell viability regarding glucose-induced apoptosis in the wild type population. Further research may be required to determine whether this resistance or lack of glucose sensitivity is linked to YMR144W for more conclusive results.
Heat Shock
| Time (sec) | Colonies | |
|---|---|---|
| 0 | >100 | |
| 15 | 92 | |
| 45 | 86 | |
| 75 | 100 | |
| 105 | 84 | |
| 135 | 81 |
| Time (sec) | Colonies | |
|---|---|---|
| 0 | 24 | |
| 15 | 20 | |
| 45 | 17 | |
| 75 | 16 | |
| 105 | 18 | |
| 135 | 14 |
Analysis
The general trend of our data yielded a negative relationship between time of heat shock. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died. Only a few of the YMR144W yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. The Wild Type yeast cells were dying off at a faster rate over time compared to the YMR144W strain. This means that the gene that was knocked out in the YMR144W yeast is more senstitive to heat shock, because without that gene it is more resistant to the heat shock.
Bases
Analysis Yeast 1, A4 A5 A6, showed the most similar growth trend in the basic environment compared to the wild type yeast, A1 A2 A3 over the 24 hour reading period. This knockout did not seem to be affected by the ammonium chloride and ammonium hydroxide buffer at a pH 8.5.
Protocol- UW-Stout/Bases SP23
Electrical Shock
Protocol
Data
Analysis
These two pictures show how the knockout strain reacts with either no electrical shock or an electrical shock of 2.5 kV. These were the same experiments but on different days to make sure things looked like each other. With this first knockout strain, we see that these acted just like our wild type cells. They show a good number of cells on our negative control, but when we shocked them at 2.5kV, many died. With our second trial we noticed that we had contamination in all of our plates. While our cells did grow so did other things.
References
See Help:References on how to add references
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
