YGL146C
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| Systematic name | YGL146C |
| Gene name | RRT6 |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VII:229686..228751 |
| Primary SGDID | S000003114 |
Description of YGL146C: Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins[1][2][3]
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Contents
Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW Stout/D2O SP22
The YGL146C Knock Out Yeast Strain was A2 and definitely unaffected by the 35% D20 dilution. It showed when it stayed in a maximum range of 0.4-0.5 OD 600.
UW Stout/Sucrose Fermentation SP22
| Gene | Glucose | Fructose | Ethanol |
| Standard solution | 2.0000 | 0.2000 | 2.0000 |
| YDl109C | 0.3800 | 0.3933 | 0.3430 |
| YGL140C | 0.2212 | 0.2685 | 0.1867 |
| YOR111W | 0.3332 | 0.3598 | 0.1343 |
| YHL029C | 0.3870 | 0.2368 | 0.1151 |
| YDR307W | 0.4366 | 0.2487 | 0.0606 |
| YNL058C | 0.2710 | 0.3056 | 0.1577 |
| YCL049C | |||
| YGR079W | |||
| YBL113C | 0.3498 | 0.2012 | 0.1434 |
| BY4735 | 0.3171 | 0.3084 | 0.3541 |
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UW-Stout/UV Light SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.
RESULTS
INTERPERTATION
In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 95% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
References
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- ↑ Hontz RD, et al. (2009) Genetic Identification of Factors That Modulate Ribosomal DNA Transcription in Saccharomyces cerevisiae. Genetics 182(1):105-19 SGD PMID 19270272
- ↑ Pearson BM and Schweizer M (2002) Basic functional analysis of six unknown open reading frames from Saccharomyces cerevisiae: four from chromosome VII and two from chromosome XV. Yeast 19(2):123-9 SGD PMID 11788967
- ↑ Voet M, et al. (1997) The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13(2):177-82 SGD PMID 9046099
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