YGL146C

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Systematic name YGL146C
Gene name RRT6
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VII:229686..228751
Primary SGDID S000003114


Description of YGL146C: Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins[1][2][3]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW Stout/D2O SP22

4.28.2022 1.png

The YGL146C Knock Out Yeast Strain was A2 and definitely unaffected by the 35% D20 dilution. It showed when it stayed in a maximum range of 0.4-0.5 OD 600.


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UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene1.jpg


INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 90% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.

References

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  1. Hontz RD, et al. (2009) Genetic Identification of Factors That Modulate Ribosomal DNA Transcription in Saccharomyces cerevisiae. Genetics 182(1):105-19 SGD PMID 19270272
  2. Pearson BM and Schweizer M (2002) Basic functional analysis of six unknown open reading frames from Saccharomyces cerevisiae: four from chromosome VII and two from chromosome XV. Yeast 19(2):123-9 SGD PMID 11788967
  3. Voet M, et al. (1997) The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13(2):177-82 SGD PMID 9046099

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