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Systematic name YCL002C
Gene name
Feature type ORF, Uncharacterized
Coordinates Chr III:111675..110808
Primary SGDID S000000508

Description of YCL002C: Putative protein of unknown function; YCL002C is not an essential gene[1]

Community Commentary

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UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YCLO02C on synthetic defined media (SDC).
Growth of YCLO02C on omission media that lacks nitrogen.

Knocking out the YCLO02C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YCL002C uv light.jpg.jpg BY4735(wild) uv light.jpg

YCL002C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Rate in YPD

ycl002c growth.png

In a BY4735 background, knocking out YCL002C seems to have no effect on growth rate in log-phase in YPD. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCL002C knock-out strain's doubling time was 134 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.


Methanol Sensitivity

The wild type had a doubling time of 175 minutes, the YCL002 had a doubling time of 315 minutes. The addition of methanol sped up our reaction and caused the yeast cells to multiply faster.


YCL002C Methanol.png

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCL002C also portrayed a small difference in doubling time, indicating that this gene most likely does not play a role in DNA replication.

HU YCL002C.png

Stressing with different pH's

pH stress protocol link [2] - The wild type strain had a significant difference in doubling time when my partner and I stressed it out with a varying levels of acidic pH's and It displayed that when we tested it on the final lab experiment of a pH of 4 that it was highly sensitive to that particular pH and In some of the test conditions that it displayed that they have died or are very very slowly growing. But the yeast gene strain YCL002C we came to the conclusion that it was a lot more resilient to the acidic pH of 4 than the wild type strain.

YCL002 data.PNG


See Help:References on how to add references

  1. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549

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