YBL113C

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Systematic name	YBL113C
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr II:2658..280
Primary SGDID	S000002153

Description of YBL113C: Helicase-like protein encoded within the telomeric Y' element




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW Stout/D2O SP22

In the results of the 35% dilution of D2O with the Knock-out Yeast Strain YBL113C (A9) we can see that the growth curve stayed in the 0.1-0.3 range throughout the data collected in 24 hours. This tells us it was more sensitive to the dilution of D2O.

UW-Stout/Heat Shock SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.

Results

Interpretation

From the data gathered, there was a medium positive effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were 252% more resistant to heat shock than the control.

UW Stout/Sucrose Fermentation SP22

Gene	Glucose	Fructose	Ethanol
Standard solution	2.0000	0.2000	2.0000
YDl109C	0.3800	0.3933	0.3430
YGL140C	0.2212	0.2685	0.1867
YOR111W	0.3332	0.3598	0.1343
YHL029C	0.3870	0.2368	0.1151
YDR307W	0.4366	0.2487	0.0606
YNL058C	0.2710	0.3056	0.1577
YCL049C	0.4078	0.3052	0.1969
YGR079W	0.4042	0.1589	0.0080
YBL113C	0.3498	0.2012	0.1434
BY4735	0.3171	0.3084	0.3541

Interpretation

This gene produced 40.50% of the amount of ethanol that the wild type produced.

UW-Stout/Caffeine SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 4mM of caffeine.

Results

BY4735(wild type yeast) and YBL113C(knockout yeast gene) growth after being subjected to 4mM caffeine solution.

Interpretation

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UW-Stout/Hydrogen Peroxide SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to hydrogen peroxide.

Results

BY4735 and YBL113C after being exposed to 0.065% dilution of hydrogen peroxide solution.

Interpretation

From the growth curve, we can see that the knockout strain followed a similar sensitivity pattern to the wild type strain, suggesting that the grow has inhibited identically.

References

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UW-Stout/Nonionic Detergent SP22

-According to the graph above, we can conclude that the KO yeast cell YBL113C follow a smaller sensitivity to the stress than the Wild type strain.