YHL029C
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Systematic name | YHL029C |
Gene name | OCA5 |
Aliases | |
Feature type | ORF, Uncharacterized |
Coordinates | Chr VIII:47968..45929 |
Primary SGDID | S000001021 |
Description of YHL029C: Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts[1][2]
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Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW Stout/D2O SP 22
The YHL029C Knock Out Yeast Strain (A4) is unaffected by the 35% dilution D2O . With the maximum optical density of about 0.5-0.6 OD 600.
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UW-Stout/UV Light SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.
RESULTS
INTERPERTATION
In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
References
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- ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
- ↑ Kushner DB, et al. (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9 SGD PMID 14671320
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