YMR221C

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Systematic name	YMR221C
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr XIII:715445..713931
Primary SGDID	S000004834

Description of YMR221C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Heat Shock FA21

YMR221C (C)

• When stressing this strain of yeast, there was not much of a growth difference. The growth rate slowed down once heat shocked.

Nitrogen Starvation

• In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.

UW-Stout/Bud Scars FA21

Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YMR221C cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells.

pH

YMR221C

• When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain

Protocol: [[1]]

UW-Stout/G418 FA21

The YMR221C yeast strain doubling time without any stress was 172 minutes which is very similar to the wild type with 182 minutes. When both strains were stressed by G418 they YMR221C doubling time was 184 minutes and the wild type was 178 minutes. (These times are averaged between three trials)

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