YGL235W
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| Systematic name | YGL235W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VII:55279..55815 |
| Primary SGDID | S000003204 |
Description of YGL235W: Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2[1][2]
Contents
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Protein Details
Protein Modification
Modification(s): Phosphorylation
Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [2] [3]
UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YGL235Wseems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
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YGL235W is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YGL235W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YGL235W knock-out strain's doubling time was 209 minutes.
G-418 Stress
In the BY4735 background, knocking out YGL235W seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YGL235W knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YGL235W knock-out strain's doubling time was 93 minutes.
Methanol Sensitivity
The wild type had a doubling time of 160 min, the YGL235w had a doubling time of 160 min. The strain was sensitive to the methanol.
Stressing with Hydroxyurea
Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YGL235W had about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.
Fermentation
Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
References
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- ↑ Chamilos G, et al. (2008) Genomewide Screening for Genes Associated with Gliotoxin Resistance and Sensitivity in Saccharomyces cerevisiae. Antimicrob Agents Chemother 52(4):1325-9 SGD PMID 18212113
- ↑ 2.0 2.1 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
SGD PMID 14574415 Cite error: Invalid
<ref>tag; name "S000074306" defined multiple times with different content - ↑ submitted by Jeff Ubersax on 2004-01-27
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