Difference between revisions of "YHL029C"
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| + | This gene produced 32.50% of the amount of ethanol that the wild type produced. | ||
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Revision as of 12:36, 3 May 2022
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| Systematic name | YHL029C |
| Gene name | OCA5 |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VIII:47968..45929 |
| Primary SGDID | S000001021 |
Description of YHL029C: Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts[1][2]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW Stout/D2O SP 22
The YHL029C Knock Out Yeast Strain (A4) is unaffected by the 35% dilution D2O . With the maximum optical density of about 0.5-0.6 OD 600.
UW Stout/Sucrose Fermentation SP22
| Gene | Glucose | Fructose | Ethanol |
| Standard solution | 2.0000 | 0.2000 | 2.0000 |
| YDl109C | 0.3800 | 0.3933 | 0.3430 |
| YGL140C | 0.2212 | 0.2685 | 0.1867 |
| YOR111W | 0.3332 | 0.3598 | 0.1343 |
| YHL029C | 0.3870 | 0.2368 | 0.1151 |
| YDR307W | 0.4366 | 0.2487 | 0.0606 |
| YNL058C | 0.2710 | 0.3056 | 0.1577 |
| YCL049C | |||
| YGR079W | |||
| YBL113C | 0.3498 | 0.2012 | 0.1434 |
| BY4735 | 0.3171 | 0.3084 | 0.3541 |
Interpretation
This gene produced 32.50% of the amount of ethanol that the wild type produced.
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UW-Stout/UV Light SP22
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.
RESULTS
INTERPERTATION
In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
References
See Help:References on how to add references
- ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
- ↑ Kushner DB, et al. (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9 SGD PMID 14671320
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
