Difference between revisions of "YGL146C"

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The YGL146C Knock Out Yeast Strain was A2 and definitely unaffected by the 35% D20 dilution. It showed when it stayed in a maximum range of 0.4-0.5 OD 600.
 
The YGL146C Knock Out Yeast Strain was A2 and definitely unaffected by the 35% D20 dilution. It showed when it stayed in a maximum range of 0.4-0.5 OD 600.
  
 +
===[[UW Stout/Sucrose Fermentation SP22]]===
 +
 +
{| border="1" cellspacing="4" cellpadding="2"
 +
|Gene
 +
|Glucose
 +
|Fructose
 +
|Ethanol
 +
|-
 +
|Standard solution
 +
|2.0000
 +
|0.2000
 +
|2.0000
 +
|-
 +
|YDl109C
 +
|0.3800
 +
|0.3933
 +
|0.3430
 +
|-
 +
|YGL140C
 +
|0.2212
 +
|0.2685
 +
|0.1867
 +
|-
 +
|YOR111W
 +
|0.3332
 +
|0.3598
 +
|0.1343
 +
|-
 +
|YHL029C
 +
|0.3870
 +
|0.2368
 +
|0.1151
 +
|-
 +
|YDR307W
 +
|0.4366
 +
|0.2487
 +
|0.0606
 +
|-
 +
|YNL058C
 +
|0.2710
 +
|0.3056
 +
|0.1577
 +
|-
 +
|YCL049C
 +
|
 +
|
 +
|
 +
|-
 +
|YGR079W
 +
|
 +
|
 +
|
 +
|-
 +
|YBL113C
 +
|0.3498
 +
|0.2012
 +
|0.1434
 +
|-
 +
|BY4735
 +
|0.3171
 +
|0.3084
 +
|0.3541
 +
|}
  
 
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Revision as of 12:23, 3 May 2022

Share your knowledge...Edit this entry! <protect>

Systematic name YGL146C
Gene name RRT6
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VII:229686..228751
Primary SGDID S000003114


Description of YGL146C: Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins[1][2][3]




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Community Commentary

About Community Commentary. Please share your knowledge!


This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW Stout/D2O SP22

4.28.2022 1.png

The YGL146C Knock Out Yeast Strain was A2 and definitely unaffected by the 35% D20 dilution. It showed when it stayed in a maximum range of 0.4-0.5 OD 600.

UW Stout/Sucrose Fermentation SP22

Gene Glucose Fructose Ethanol
Standard solution 2.0000 0.2000 2.0000
YDl109C 0.3800 0.3933 0.3430
YGL140C 0.2212 0.2685 0.1867
YOR111W 0.3332 0.3598 0.1343
YHL029C 0.3870 0.2368 0.1151
YDR307W 0.4366 0.2487 0.0606
YNL058C 0.2710 0.3056 0.1577
YCL049C
YGR079W
YBL113C 0.3498 0.2012 0.1434
BY4735 0.3171 0.3084 0.3541

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UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene2.jpg


INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 95% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.

References

See Help:References on how to add references

  1. ↑ Hontz RD, et al. (2009) Genetic Identification of Factors That Modulate Ribosomal DNA Transcription in Saccharomyces cerevisiae. Genetics 182(1):105-19 SGD PMID 19270272
  2. ↑ Pearson BM and Schweizer M (2002) Basic functional analysis of six unknown open reading frames from Saccharomyces cerevisiae: four from chromosome VII and two from chromosome XV. Yeast 19(2):123-9 SGD PMID 11788967
  3. ↑ Voet M, et al. (1997) The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13(2):177-82 SGD PMID 9046099

See Help:Categories on how to add the wiki page for this gene to a Category </protect>

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