Difference between revisions of "YBR225W"
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Revision as of 13:27, 27 April 2021
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| Systematic name | YBR225W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr II:670627..673329 |
| Primary SGDID | S000000429 |
Description of YBR225W: Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components[1][2]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YBR225W does not seem to have any effect on the strain's growth rate. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YBR225W knock-out strain's doubling time was 156 minutes. (These doubling times are the means of three experiments.)
Hydroxychloroquine
HCQ refers to hydroxychloroquine
Under normal conditions, the BY4753 doubling time is 144 minutes, while that of YBR225W is 236 minutes. Thus, YBR225W doubles slower than the wild strain. When grown in an environment with hydroxychloroquine, YBR225W doubled in 191 minutes, while BY4753 doubled in 276 minutes. Surprisingly, YBR225W doubling time decreased dramatically, which means that it grew faster in the presence of hydroxychloroquine. The BY4753 strain was inhibited by hydroxychloroquine. (These doubling times and curves are the means of three experiments.)
Salt Concentration (NaCl)
0mM NaCl conc. BY4735 strain's doubling time: 169 minutes
0mM NaCl conc. YBR225W strain's doubling time: 224 minutes
750mM NaCl conc. BY4735 strain's doubling time: 294 minutes
750mM NaCl conc. YBR225W strain's doubling time: 226 minutes
The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Knocking out the gene negatively impacted the growth rate. Comparing the YBR225W doubling times in 0mM and 750mM NaCl, there is a no effect on the growth rate. This effect has no influence on the knockout strain's (YBR225W) growth.
Caffeine Group 1
When adding caffeine to the YBR225W strain the growth rate had a moderate effect. The doubling time of the strains were as followed:
Doubling time of YBR225W with caffeine: 296 minutes
Doubling time of YBR225W without caffeine: 208 minutes
Doubling time of BY4735 with caffeine: -2649 minutes
Doubling time of BY4735 without caffeine: 187 minutes
The effect that the caffeine had on the YBR225W knockout strain increased the doubling time. The effect was it made the yeast grow at a slower rate.
Competative Co-culture
These are the results of a competitive co-culture protocol. The knockout strain was grown in a culture to test fitness against a green, fluorescent wild-type strain. The percentage of analyzed cells in the culture measured was those displaying green fluorescence. In theory, this should mean if a knockout has reduced the fitness of a strain of yeast, the fluorescent wild-type strain would have a higher percentage than the knockout strain. If the knockout does not decrease fitness, they would be roughly equal. This may not be so in results. Sources of error may include contamination and human error.
The results here are not expected. It was not readily apparent this knockout strain grew quickly, so more likely than not, both tests were contaminated with an organism that grows faster than yeast and does not display a fluorescent trait like the wild-type.
Ammonium Sulfate
References
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