Difference between revisions of "YBL081W"
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| − | As we can tell from the figure above that Test 1 did not do well under the stress but tests 2-4 were very happy. The growth Models 1-3 are what the yeasts growth model is not | + | As we can tell from the figure above that Test 1 did not do well under the stress but tests 2-4 were very happy. The growth Models 1-3 are what the yeasts growth model is not stressed. |
==Ethanol Tolerance in YBL081W== | ==Ethanol Tolerance in YBL081W== | ||
Revision as of 13:57, 12 December 2019
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| Systematic name | YBL081W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr II:71866..72972 |
| Primary SGDID | S000000177 |
Description of YBL081W: Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport[1][2]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth in YPD
In a BY4735 background, knocking out YBL081W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YAL061 knock-out strain's doubling time was 519 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 741.26 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
This bar graph show that compared to a BY4735 backgroung, Knocking out yBL081w make yeast more sensitive to UV light exposure
Cycloheximide on Yeast strand YBL081W
As we can tell from the figure above that Test 1 did not do well under the stress but tests 2-4 were very happy. The growth Models 1-3 are what the yeasts growth model is not stressed.
Ethanol Tolerance in YBL081W
This graph shows the growth of YBL081 treated and untreated with Ethanol. The strands of YBL081 that were treated with ethanol had an increase in growth compared to those that weren't. The doubling time of YBL081 that was treated with ethanol is (answer). And the doubling time of the strands that weren't is (answer). This shows that Ethanol leads to an increase in growth in YBL081W.
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References
See Help:References on how to add references
- ↑ Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
- ↑ Herst PM, et al. (2008) Plasma membrane electron transport in Saccharomyces cerevisiae depends on the presence of mitochondrial respiratory subunits. FEMS Yeast Res 8(6):897-905 SGD PMID 18657191
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