Difference between revisions of "YMR090W"

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-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YMR090W showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays a role in DNA replication.  
 
-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YMR090W showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays a role in DNA replication.  
 
[[file:HU_YMR090W.png|left]]
 
[[file:HU_YMR090W.png|left]]
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===Fermentation===
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Fermentation protocol link [https://wiki.yeastgenome.org/index.php/UW-Stout/Fermentation]
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- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
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[[File:Fer YMR090W.PNG]]
  
 
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Revision as of 08:46, 5 May 2019

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Systematic name YMR090W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XIII:449245..449928
Primary SGDID S000004696


Description of YMR090W: Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability[1][2][3]




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UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YMRO90W on synthetic defined media (SDC).
Growth of YMRO90W on omission media that lacks nitrogen.

Knocking out the YMRO90W seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YMR090W uv light.jpg BY4735(wild) uv light.jpg

YPL068C is more sensitive than YMR090W given that there is less colonizes present when under the same stress.


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically higher expression in nitrogen limited chemostat cultures versus nitrogen excess. [4] [5]

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

ymr090w growth.png

In a BY4735 background, knocking out YMR090W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YMR090W knock-out strain's doubling time was 208 minutes.

image002.png

Methanol Sensitivity

The wild type had a doubling time of 170 min, the YMR090w had a doubling time of 225 min. The doubling time increased for both after we added the methanol which means that this strain is sensitive.

[1]

YMR090W Methanol sensitivity.png

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YMR090W showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays a role in DNA replication.

HU YMR090W.png

Fermentation

Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Fer YMR090W.PNG


References

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  1. ↑ Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
  2. ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  3. ↑ Santos PM, et al. (2009) Insights into yeast adaptive response to the agricultural fungicide mancozeb: a toxicoproteomics approach. Proteomics 9(3):657-70 SGD PMID 19137554
  4. ↑ Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  5. ↑ submitted by Viktor Boer on 2003-07-25

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