Difference between revisions of "YJL043W"
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-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YJL043W showed about a 20 minute difference of doubling time from the wild type strain when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication. | -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YJL043W showed about a 20 minute difference of doubling time from the wild type strain when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication. | ||
+ | [[file:HU_YJLO43W.png|left]] | ||
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Revision as of 19:55, 2 May 2019
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Systematic name | YJL043W |
Gene name | |
Aliases | |
Feature type | ORF, Uncharacterized |
Coordinates | Chr X:360130..360903 |
Primary SGDID | S000003579 |
Description of YJL043W: Putative protein of unknown function; YJL043W is a non-essential gene[1][2]
Contents
Community Commentary
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UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YJL043W seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
YJL043W is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YJL043W seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YJL043W knock-out strain's doubling time was 155 minutes.
Methanol Sensitivity
Stressing with Hydroxyurea
Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YJL043W showed about a 20 minute difference of doubling time from the wild type strain when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.
References
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- ↑ Entian KD, et al. (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702 SGD PMID 10628851
- ↑ Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
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