Difference between revisions of "YHL029C"

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(UW Stout/D2O SP 22)
(Community Commentary)
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===[[UW-Stout/UV Light SP22]]===
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As part of the University of Wisconsin Stout [[UW-STOUT/About|Orphan Gene Project]] this gene was tested under a UV Light [[UW-Stout/UV Light SP22|using this protocol.]]
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'''RESULTS'''
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[[File:ASHTONMIKOLOSKIFinal Project Graph.png|400px|]]
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[[File:AshtonGene4.jpg|400px|]]
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'''INTERPERTATION'''
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In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.
  
 
==References==
 
==References==

Revision as of 11:35, 3 May 2022

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Systematic name YHL029C
Gene name OCA5
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:47968..45929
Primary SGDID S000001021


Description of YHL029C: Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts[1][2]




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Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW Stout/D2O SP 22

4.28.2022 1.png

The YHL029C Knock Out Yeast Strain (A4) is unaffected by the 35% dilution D2O . With the maximum optical density of about 0.5-0.6 OD 600.



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UW-Stout/UV Light SP22

As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested under a UV Light using this protocol.

RESULTS

ASHTONMIKOLOSKIFinal Project Graph.png AshtonGene4.jpg


INTERPERTATION In the graph and photos above, exposing this gene to 600 seconds of 400 Watt UV Light killed approximately 75% of yeast cell cultures, compared to its control counterpart, which was the same gene and amount of cells, just was not exposed to UV Light.

References

See Help:References on how to add references

  1. Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  2. Kushner DB, et al. (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9 SGD PMID 14671320

See Help:Categories on how to add the wiki page for this gene to a Category </protect>